Bielawska-Drózd Agata, Cieślik Piotr, Bohacz Justyna, Korniłłowicz-Kowalska Teresa, Żakowska Dorota, Bartoszcze Michał, Wlizło-Skowronek Bożena, Winnicka Izabela, Brytan Marek, Kubiak Leszek, Skopińska-Różewska Ewa, Kocik Janusz
Biological Threats Identification and Countermeasure Centre, Military Institute of Hygiene and Epidemiology, Puławy, Poland.
Faculty of Agrobioengineering, Department of Environmental Microbiology, Laboratory of Mycology, University of Life Sciences in Lublin, Lublin, Poland.
Ann Agric Environ Med. 2018 Jun 20;25(2):274-279. doi: 10.26444/aaem/80711. Epub 2018 Jan 25.
The goal of the study was a microbiological, qualitative and quantitative analysis of bioaerosol at the workplace of medical personnel (Health Emergency Departments (HEDs), ambulances), and comparative administration offices with an expected neutral occupational exposure to biological agents measured with individual Button Sampler.
Personal sampling was performed with Button Sampler instrument loaded with gelatine filters in 10 HEDs, in 9 ambulances and in 9 offices to assess the occupational biological agents' exposure in air. Sampling was conducted from March until April 2016. Samples were quantitatively assessed for viable and total number of bacteria and fungi. Routine procedures for microbiological diagnostics were implemented. Data were analysed using Kruskal-Wallis and Mann-Whitney statistical tests with α=0.05. P value less than 0.05 were considered significant.
At the workplaces assessed, the concentrations of viable microorganisms in HEDs were 1.3×10 - 4.2×10 CFU/m for bacteria, 3.4×10 - 8.1×10 CFU/m for fungi; in ambulances 1.3×10 - 1.4×10 CFU/m (bacteria), 6.7×10 - 6.5×10 CFU/m (fungi) and in offices 4.2×10 - 5.0×10 CFU/m (bacteria), 0 - 7.9×10 CFU/m(fungi). In outdoor air, the number of microorganisms reached the level: 1.0×10 - 5.9×10 CFU/m for bacteria and 1.5×10 - 8.2×10 CFU/m for fungi. The predominant isolated bacteria were Gram-positive cocci. The prevalent fungi species belonged to the genus and .
The quantitative assessment of examined indoor air was similar to control outdoor air, and were relatively low. The level of microbiological contamination did not exceed 5×10 CFU/m which is recommended as an admissible level in public spaces in Poland.
本研究的目的是对医务人员工作场所（健康急诊科（HEDs）、救护车）以及预期职业性生物制剂暴露为中性的行政办公室中的生物气溶胶进行微生物学、定性和定量分析，采用个体纽扣采样器进行测量。
在10个健康急诊科、9辆救护车和9间办公室中，使用装有明胶滤膜的纽扣采样器进行个人采样，以评估空气中职业性生物制剂的暴露情况。采样时间为2016年3月至4月。对样品中的活菌和细菌及真菌总数进行定量评估。实施微生物诊断的常规程序。使用Kruskal-Wallis和Mann-Whitney统计检验分析数据，α = 0.05。P值小于0.05被认为具有显著性。
在所评估的工作场所中，健康急诊科中活菌微生物浓度为细菌1.3×10 - 4.2×10 CFU/m，真菌3.4×10 - 8.1×10 CFU/m；救护车中为细菌1.3×10 - 1.4×10 CFU/m，真菌6.7×10 - 6.5×10 CFU/m；办公室中为细菌4.2×10 - 5.0×10 CFU/m，真菌0 - 7.9×10 CFU/m。在室外空气中，微生物数量达到以下水平：细菌1.0×10 - 5.9×10 CFU/m，真菌1.5×10 - 8.2×10 CFU/m。分离出的主要细菌为革兰氏阳性球菌。流行的真菌种类属于属和。
所检测的室内空气定量评估结果与对照室外空气相似，且相对较低。微生物污染水平未超过5×10 CFU/m，这是波兰公共场所推荐的可接受水平。
