Lis D O, Pastuszka J S, Górny R L
Zakład Higieny Mieszkań, Instytut Medycyny Pracy, Sosnowiec.
Rocz Panstw Zakl Hig. 1997;48(1):59-68.
Quantitative criteria of microbiological air quality in homes and offices are needed for practical reasons. The purpose of this study was to obtain the preliminary characteristic of bacterial and fungal aerosols in healthy buildings. It was analysed the concentrations levels and size distributions of the investigated bioaerosols. The obtained data can be treated as a first step in the direction of determining so called normal level for different bioaerosols in homes and offices in Poland. The concentrations of airborne bacteria and fungi were measured using 6-stage Andersen impactor. The Trypcase Soy Agar were applied for bacteria and 2% Malt Extract Agar for fungi. The bacteria samples were incubated for 2 days at 37 degrees C and the fungi samples respectively for 4 days at 25 degrees C. The indoor levels of bacterial aerosol (homes: 212-888 cfu/m3, offices: 136-542 cfu/m3) were higher than the outdoor levels (respectively: 42-386 cfu/m3 and 13-115 cfu/m3). The fungal aerosol concentrations were lower indoors (homes: 81-383 cfu/m3, offices: 18-133 cfu/m3) than outdoors (94-625 cfu/m3). There were distinctly higher concentrations of the investigated bioaerosols in homes than in offices. The aerodynamic diameter of most bacterial particles were higher than 7 microns, both in indoor air (homes: 57-260 cfu/m3, offices 19-118 cfu/m3) and outdoor air (respectively: 9-145 cfu/m3 and 0-71 cfu/m3). The maximum for fungal spore levels were observed in the size range 3.3-4.7 and 2.1-3.3 microns in the instance of homes (respectively: 12-155 cfu/m3 and 19-154 cfu/m3) and in the size range 2.1-3.3 microns in the instance of offices (6-55 cfu/m3). Largest numbers of this bioaerosol in outdoor air were isolated in the size range 2.1-3.3 microns (25-208 cfu/m3). Although there are some proposals for an upper limit of the normal indoor concentration of airborne bacteria and fungi, but due to different climate and housing conditions we can expect other normal range of fungal and bacterial aerosol in Polish homes.
出于实际原因,需要制定家庭和办公室微生物空气质量的定量标准。本研究的目的是获取健康建筑中细菌和真菌气溶胶的初步特征。分析了所研究生物气溶胶的浓度水平和粒径分布。所获得的数据可被视为确定波兰家庭和办公室中不同生物气溶胶所谓正常水平方向上的第一步。使用六级安德森撞击器测量空气中细菌和真菌的浓度。用胰蛋白胨大豆琼脂培养细菌,用2%麦芽提取物琼脂培养真菌。细菌样本在37℃下培养2天,真菌样本在25℃下培养4天。室内细菌气溶胶水平(家庭:212 - 888 cfu/m³,办公室:136 - 542 cfu/m³)高于室外水平(分别为:42 - 386 cfu/m³和13 - 115 cfu/m³)。室内真菌气溶胶浓度(家庭:81 - 383 cfu/m³,办公室:18 - 133 cfu/m³)低于室外(94 - 625 cfu/m³)。家庭中所研究的生物气溶胶浓度明显高于办公室。大多数细菌颗粒的空气动力学直径大于7微米,在室内空气中(家庭:57 - 260 cfu/m³,办公室19 - 118 cfu/m³)和室外空气中(分别为:9 - 145 cfu/m³和0 - 71 cfu/m³)都是如此。在家庭中,真菌孢子水平的最大值出现在3.3 - 4.7微米和2.1 - 3.3微米的粒径范围内(分别为:12 - 155 cfu/m³和19 - 154 cfu/m³),在办公室中出现在2.1 - 3.3微米的粒径范围内(6 - 55 cfu/m³)。室外空气中这种生物气溶胶数量最多的粒径范围是2.1 - 3.3微米(25 - 208 cfu/m³)。虽然对于空气中细菌和真菌的正常室内浓度上限有一些提议,但由于气候和居住条件不同,我们预计波兰家庭中真菌和细菌气溶胶的正常范围会有所不同。