State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing Road 288, Tianjin 300020, China.
Biodynamic Optical Imaging Center, College of Life Sciences, Peking University, Beijing 100871, China.
Stem Cell Reports. 2018 Jul 10;11(1):274-287. doi: 10.1016/j.stemcr.2018.05.017. Epub 2018 Jun 21.
Transforming growth factor β1 (TGF-β1) plays a role in the maintenance of quiescent hematopoietic stem cells (HSCs) in vivo. We asked whether TGF-β1 controls the cell cycle status of HSCs in vitro to enhance the reconstitution activity. To examine the effect of TGF-β1 on the HSC function, we used an in vitro culture system in which single HSCs divide with the retention of their short- and long-term reconstitution ability. Extensive single-cell analyses showed that, regardless of its concentration, TGF-β1 slowed down the cell cycle progression of HSCs but consequently suppressed their self-renewal potential. Cycling HSCs were not able to go back to quiescence with TGF-β1. This study revealed a negative role of TGF-β1 in the regulation of the HSC number and reconstitution activity.
转化生长因子 β1（TGF-β1）在体内维持静止造血干细胞（HSCs）中发挥作用。我们想知道 TGF-β1 是否控制 HSCs 在体外的细胞周期状态，以增强重建活性。为了研究 TGF-β1 对 HSC 功能的影响，我们使用了一种体外培养系统，其中单个 HSCs 分裂时保留其短期和长期重建能力。广泛的单细胞分析表明，无论浓度如何，TGF-β1 都会减缓 HSC 的细胞周期进程，但随后会抑制其自我更新能力。TGF-β1 作用下的循环 HSCs 无法恢复静止状态。这项研究揭示了 TGF-β1 在调节 HSC 数量和重建活性方面的负面作用。
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