Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin, China.
School of Life Sciences, Jilin University, Changchun 130012, Jilin, China.
Aging (Albany NY). 2024 Jan 3;16(1):169-190. doi: 10.18632/aging.205358.
Shenzhu Erkang Syrup (SZEK) is a traditional Chinese medicine that improves spleen and stomach function, tonifying the Qi and activating the blood; however, its therapeutic effects in hematopoietic dysfunction and their underlying mechanism remain unexplored. In this study, mice were given cyclophosphamide (100 mg/kg) by intraperitoneal injections for three days to produce hematopoietic dysfunction model. We investigated the hematopoietic effect and mechanism of SZEK in mice with hematopoietic dysfunction via histopathological examination, flow cytometry, enzyme-linked immunosorbent assay, and Western blotting combined with intestinal flora and serum metabolomics analysis. In mice with hematopoietic dysfunction, SZEK (gavage, 0.3 mL/25 g) alleviated pathological damage to the bone marrow and spleen; increased the number of naïve cells (Lin), hematopoietic stem cells (LinSca-1c-Kit), long-term self-renewing hematopoietic stem cells (LinSca-1c-KitCD48CD150), B lymphocytes (CD45CD19), and macrophages (CD11bF4/80) in the bone marrow; and reduced inflammation. Preliminary intestinal flora and serum metabolome analyses indicated that the pro-hematopoietic mechanism of SZEK was associated with macrophage differentiation. Further validation revealed that SZEK promoted hematopoiesis by decreasing the number of M2 macrophages and inhibiting the secretion of negative hematopoietic regulatory factors in mice with hematopoietic dysfunction.
参术儿康糖浆是一种具有健脾和胃、益气养血作用的中药，但它在造血功能障碍方面的治疗效果及其作用机制尚不清楚。本研究采用腹腔注射环磷酰胺（100mg/kg）连续 3 天建立造血功能障碍模型，通过组织病理学检查、流式细胞术、酶联免疫吸附试验和 Western blot 结合肠道菌群和血清代谢组学分析，探讨参术儿康糖浆对造血功能障碍小鼠的造血作用及其机制。结果表明，参术儿康糖浆（灌胃，0.3mL/25g）可缓解骨髓和脾脏的病理损伤，增加骨髓中幼稚细胞（Lin）、造血干细胞（LinSca-1c-Kit）、长期自我更新造血干细胞（LinSca-1c-KitCD48CD150）、B 淋巴细胞（CD45CD19）和巨噬细胞（CD11bF4/80）的数量，减少炎症。初步的肠道菌群和血清代谢组学分析表明，参术儿康糖浆的促造血作用与巨噬细胞分化有关。进一步验证发现，参术儿康糖浆通过减少造血功能障碍小鼠 M2 型巨噬细胞的数量和抑制负向造血调控因子的分泌来促进造血。
