Nakazawa T, Hamaguchi S, Kyono-Hamaguchi Y
J Natl Cancer Inst. 1985 Sep;75(3):567-73.
Liver tumor nodules were induced by the administration of diethylnitrosamine [(DENA) CAS: 55-18-5; N-nitrosodiethylamine] to the fish, Oryzias latipes. A histochemical study showed decreased ATPase and glucose 6-phosphatase (Glc-6-Pase) activities in most of the tumor nodules. Increased ATPase staining and, occasionally, Glc-6-Pase staining were also observed. In some nodules, the distribution of bile canaliculi was disordered, indicating positive ATPase activity. Basophilic and eosinophilic nodules could be discriminated by histologic examination. The observations on serial histochemical and histologic sections revealed extreme heterogeneity in the phenotypes of the nodules. Measurement of the enzyme-altered areas indicated that the development of nodules was more prominent in male than in female fish. Experiments with sex-reversed fish, XX males and XY females, suggested that the sex difference in the susceptibility to DENA does not result from the difference in sex chromosomes but from the difference in sexual phenotypes.
通过向青鳉鱼（Oryzias latipes）体内注射二乙基亚硝胺[（DENA），化学物质登记号：55-18-5；N-亚硝基二乙胺]诱导产生肝脏肿瘤结节。组织化学研究表明，大多数肿瘤结节中的ATP酶和葡萄糖6-磷酸酶（Glc-6-Pase）活性降低。同时也观察到ATP酶染色增加，偶尔葡萄糖6-磷酸酶染色也会增加。在一些结节中，胆小管分布紊乱，表明ATP酶活性呈阳性。通过组织学检查可以区分嗜碱性和嗜酸性结节。对连续组织化学和组织学切片的观察显示，结节的表型存在极大的异质性。酶改变区域的测量表明，雄性鱼中结节的发展比雌性鱼更为显著。对性反转鱼（XX雄性和XY雌性）进行的实验表明，对二乙基亚硝胺易感性的性别差异并非由性染色体差异导致，而是由性表型差异引起。
