Pathophysiological Department, Capital Medical University, You An Men, Beijing 100069, P.R. China.
Biofactors. 2008;34(4):303-12. doi: 10.3233/BIO-2009-1084.
To elucidate the anticancer mechanism of Huqi San by assessing the expression of G-6-Pase, SDH, ATPase and AFP in N-nitrosodiethylamine-mediated hepatocarcinogenesis in rats.
A Solt-Farber two-step test model of hepatocarcino genesis was established by diethylnitrosamine (DEN) and 2-acetylaminofluorene (AAF) in rats to investigate the modifying effects of expression of 6-glucosephosphatase (G-6-Pase), succinodehydrogenase (SDH), adenosine triphosphatase (ATPase) in N-nitrosodiethylamine-mediated hepatocarcinogenesis. Hu Qisan compounded by eight medicinal herbs was prepared in glycoprival granules with wich 0.38 g crude herbs/mL solution was prepared for administration. gamma-Glutamy-transpeptidase (gamma-GT), G-6-Pase, SDH and ATPase were immunohistochemically determined. The activity of alpha-fetoglobulin (AFP) in the livers was measured with Immunofluorescence.
Huqi San treated rats showed significant decrease in areas of gamma-GT positive foci (P< 0.001). On the other hand, the expression of G-6-Pase, SDH and ATPase has obviously altered in Huqi San treated group. The activity of AFP also significantly decreased after the treatment with Huqi San (8 g/kg body weight or 4 g/kg body weight) or total alkali of mistletoe (0.12 g/kg body weight).
Huqi San can obviously increase these activities of G-6-Pase, SDH and ATPase, and at the same time significantly decrease the expression of gamma-GT and AFP. Therefore, it can obstruct or inhibit the rat's liver preneolastic lesion induced by DEN.
通过评估 G-6-Pase、SDH、ATPase 和 AFP 在二乙基亚硝胺（DEN）诱导的大鼠肝癌发生中的表达，阐明黄芪散的抗癌机制。
采用二乙基亚硝胺（DEN）和 2-乙酰氨基芴（AAF）两步法建立大鼠肝癌发生的 Solt-Farber 模型，研究黄芪散对 N-亚硝二乙胺（DEN）诱导的肝癌发生中 G-6-Pase、琥珀酸脱氢酶（SDH）、三磷酸腺苷酶（ATPase）表达的修饰作用。由 8 种草药组成的黄芪散制成甘糖酯颗粒，制备浓度为 0.38 g 粗草药/mL 的溶液用于给药。γ-谷氨酰转肽酶（γ-GT）、G-6-Pase、SDH 和 ATPase 用免疫组化法测定。用免疫荧光法测定肝脏中α-胎球蛋白（AFP）的活性。
黄芪散治疗组大鼠的γ-GT 阳性灶面积明显减少（P<0.001）。另一方面，黄芪散治疗组 G-6-Pase、SDH 和 ATPase 的表达也明显改变。黄芪散（8 g/kg 体重或 4 g/kg 体重）或槲寄生总碱（0.12 g/kg 体重）治疗后 AFP 的活性也显著降低。
黄芪散能明显增加 G-6-Pase、SDH 和 ATPase 的活性，同时显著降低γ-GT 和 AFP 的表达。因此，它可以阻止或抑制 DEN 诱导的大鼠肝前病变。
