Guo Weiwei, Ren Lili, Chen Lei, Ning Yu, Zhao Lidong, Yang Shiming
Department of Otolaryngology-Head & Neck Surgery, Institute of Otolaryngology of PLA, Chinese PLA General Hospital, Beijing 100853, China.
State Key Laboratory for Agro-biotechnology, China Agricultural University, Beijing, China.
J Otol. 2015 Jun;10(2):83-86. doi: 10.1016/j.joto.2015.09.005. Epub 2015 Sep 30.
To identify transcript variants and expression patterns of porcine .
A pairwise BLAST search at NCBI database was performed to deduce the structure of porcine gene. Subsequently, 5' RACE and fluorescent quantitative RT-PCR were used to analyze the expression pattern of porcine in different tissues.
Four transcript variants of porcine , MITF-A, MITF-H, MITF-M and MITF-SUS were identified, all sharing high homology with those in humans, except Mitf-SUS.
The sequence of porcine appear highly homologous to human . However, only 4 transcript variants of porcine were identified in these minipigs, less than the 9 transcript variants in human .
鉴定猪的转录本变体和表达模式。
在NCBI数据库中进行成对BLAST搜索以推导猪基因的结构。随后，采用5' RACE和荧光定量RT-PCR分析猪在不同组织中的表达模式。
鉴定出猪的四种转录本变体，即MITF-A、MITF-H、MITF-M和MITF-SUS，除Mitf-SUS外，其余均与人类的高度同源。
猪的序列与人的序列高度同源。然而，在这些小型猪中仅鉴定出4种猪的转录本变体，少于人类的9种转录本变体。
