Transcript variants and expression profiles analysis of gene in minipigs.

OBJECT

To identify transcript variants and expression patterns of porcine .

MATERIALS AND METHODS

A pairwise BLAST search at NCBI database was performed to deduce the structure of porcine gene. Subsequently, 5' RACE and fluorescent quantitative RT-PCR were used to analyze the expression pattern of porcine in different tissues.

RESULTS

Four transcript variants of porcine , MITF-A, MITF-H, MITF-M and MITF-SUS were identified, all sharing high homology with those in humans, except Mitf-SUS.

CONCLUSION

The sequence of porcine appear highly homologous to human . However, only 4 transcript variants of porcine were identified in these minipigs, less than the 9 transcript variants in human .