Furuichi T, Komano T, Inouye M, Inouye S
Mol Gen Genet. 1985;199(3):434-9. doi: 10.1007/BF00330755.
Protein S is a development-specific protein of Myxococcus xanthus encoded by the tps gene. It has been shown that there are two extensively homologous genes (ops and tps) tandemly repeated in the same direction with a 1.4 kb spacer fragment between them (Inouye et al. 1983). Seven deletion mutants were constructed by removing the ops gene, the tps gene, segments of the spacer sequence or combinations of these regions. The deleted regions were replaced with DNA fragments carrying the Tn5 gene for kanamycin resistance. The effects of deleting different regions on morphological changes and on patterns of protein synthesis during fruiting body formation were examined. The process of fruiting body formation was severely delayed when both the ops and the tps genes were deleted. However, this delay could be suppressed by either the ops gene or the tps gene, individually, although in the latter case, a slight delay was still observed. These results indicate that the ops gene is expressed during fruiting body formation and plays a role in the normal program of M. xanthus differentiation. Furthermore, the role of the ops gene can be complemented by the tps gene. The deletion of the ops and/or tps genes had no effect on glycerol-spore formation.
蛋白质S是由tps基因编码的黄色粘球菌的一种发育特异性蛋白质。研究表明，有两个广泛同源的基因（ops和tps）以相同方向串联重复，它们之间有一个1.4 kb的间隔片段（Inouye等人，1983年）。通过去除ops基因、tps基因、间隔序列片段或这些区域的组合构建了七个缺失突变体。将缺失区域替换为携带卡那霉素抗性Tn5基因的DNA片段。研究了缺失不同区域对子实体形成过程中形态变化和蛋白质合成模式的影响。当ops和tps基因都缺失时，子实体形成过程严重延迟。然而，这种延迟可以被ops基因或tps基因单独抑制，尽管在后一种情况下，仍观察到轻微延迟。这些结果表明，ops基因在子实体形成过程中表达，并在黄色粘球菌分化的正常程序中发挥作用。此外，ops基因的作用可以被tps基因互补。ops和/或tps基因的缺失对甘油孢子形成没有影响。
