Komano T, Furuichi T, Teintze M, Inouye M, Inouye S
J Bacteriol. 1984 Jun;158(3):1195-7. doi: 10.1128/jb.158.3.1195-1197.1984.
A deletion mutation of the gene for protein S (tps), a development-specific protein of Myxococcus xanthus, was constructed. No significant differences in the process of fruiting body formation or the yield of myxospores were observed between mutant and wild-type cells. On the other hand, when the tps gene was deleted together with a 2.0-kilobase sequence including the ops gene immediately upstream of the tps gene, fruiting body formation was substantially delayed, and the yield of myxospores was reduced. These results indicate that protein S is not essential for differentiation of M. xanthus, whereas a gene product(s) coded from the sequence upstream of the tps gene appears to be required for normal fruiting body formation.
构建了黄色粘球菌发育特异性蛋白S（tps）基因的缺失突变体。在突变体细胞和野生型细胞之间，未观察到子实体形成过程或粘孢子产量的显著差异。另一方面，当tps基因与包括tps基因上游紧邻的ops基因的2.0千碱基序列一起缺失时，子实体形成显著延迟，并且粘孢子产量降低。这些结果表明，蛋白S对于黄色粘球菌的分化不是必需的，而tps基因上游序列编码的一种或多种基因产物似乎是正常子实体形成所必需的。
