Sokolov N N, Fitsner A B, Anikeitcheva N V, Samko O T, Kolosha V O, Fodor I, Votrin I I
Mol Biol Rep. 1985 Apr;10(3):159-61. doi: 10.1007/BF00778522.
PaeI, a new restriction endonuclease from Pseudomonas aeruginosa clinical strain was isolated and characterized. It recognizes and cleaves the sequence 5'-GCATG reduced C-3' generating DNA fragments with 3'-tetranucleotide sticky ends. DNAs of pBR322, SV40 and bacteriophage lambda have one, two and six PaeI recognition sites, respectively. Seventy-two strains of Pseudomonas, Clostridium, Escherichia coli, Shigella, Proteus and Saccharomyces were screened for the presence of site-specific endonucleases. Here we describe the PaeI restriction enzyme found in Pseudomonas aeruginosa; other data will be published elsewhere. Earlier Hinkle and Miller isolated from P. aeruginosa a PaeR7 restriction endonuclease recognizing and cleaving a sequence 5'-C reduced TCGAG-3' (1). Sequence analysis of DNAs cleaved by PaeI shows that the enzyme is the isoschizomer of SphI (2).
从铜绿假单胞菌临床菌株中分离并鉴定出一种新的限制性内切酶PaeI。它识别并切割序列5'-GCATG↓C-3'，产生具有3'-四核苷酸粘性末端的DNA片段。pBR322、SV40和噬菌体λ的DNA分别有一个、两个和六个PaeI识别位点。对72株假单胞菌、梭菌、大肠杆菌、志贺氏菌、变形杆菌和酿酒酵母菌株进行了位点特异性内切酶的筛选。在此，我们描述了在铜绿假单胞菌中发现的PaeI限制酶；其他数据将在其他地方发表。此前，欣克尔和米勒从铜绿假单胞菌中分离出一种PaeR7限制内切酶，它识别并切割序列5'-C↓TCGAG-3'（1）。PaeI切割的DNA序列分析表明，该酶是SphI的同裂酶（2）。
