一种来自钝顶螺旋藻的新型限制性内切酶。

A new restriction endonuclease from Spirulina platensis.

作者信息

Kawamura M, Sakakibara M, Watanabe T, Kita K, Hiraoka N, Obayashi A, Takagi M, Yano K

出版信息

Nucleic Acids Res. 1986 Mar 11;14(5):1985-9. doi: 10.1093/nar/14.5.1985.

DOI:10.1093/nar/14.5.1985

PMID:3008081

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339637/

Abstract

Three restriction endonucleases, Sp1I, Sp1II and Sp1III have been purified partially from Spirulina platensis subspecies siamese and named. Sp1I cleaves bacteriophage lambda DNA at one site, phi X 174 RF DNA at two sites, but does not cleave pBR322 DNA. This enzyme recognizes the sequence 5'CGTACG3' 3'GCATCG5' and cuts the site indicated by the arrows. Sp1II is an isoschizomer of Tth111I and Sp1III is an isoschizomer of HaeIII.

摘要

已从钝顶螺旋藻暹罗亚种中部分纯化出三种限制性内切酶，分别命名为Sp1I、Sp1II和Sp1III。Sp1I在一个位点切割噬菌体λ DNA，在两个位点切割φX174 RF DNA，但不切割pBR322 DNA。该酶识别序列5'CGTACG3' 3'GCATCG5'，并在箭头指示的位点切割。Sp1II是Tth111I的同裂酶，Sp1III是HaeIII的同裂酶。