Fabianová Kamila, Závodská Monika, Raček Adam, Angelidis Andreas, Martončíková Marcela, Račeková Enikő
Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4-6, 04001, Košice, Slovakia.
Gen Physiol Biophys. 2018 May;37(3):275-283. doi: 10.4149/gpb_2017056.
Accumulating evidence confirms that the exposure of neonatal rats to maternal separation can significantly alter individual processes of postnatal neurogenesis in the olfactory neurogenic region - the subventricular zone (SVZ) and the rostral migratory stream (RMS). To establish the stressful influence of MS on postnatal neurogenesis we have investigated whether altered olfactory environment caused by short-term MS induces expression of Fos protein in the SVZ/RMS and in the olfactory cortical area - anterior olfactory nucleus (AON) of neonatal rats. Pups were separated from mothers for 2 hours at the postnatal days 7, 14 and 21. Immunohistochemically labeled Fos protein was assessed. Our results revealed that single exposure to MS is a stressful event that selectively and in age-dependent manner stimulates cellular activity in the SVZ and AON. A few Fos+ cells were found in the SVZ of P21 control animals and MS significantly increased their number. This suggests that some SVZ cells are included in the circuitry, which is activated by MS and that these cells have complete equipment for the Fos signal transduction. MS significantly increased the number of Fos+ cells in the AON in all age stages examined suggesting that its effect is mediated by olfaction.
越来越多的证据证实，新生大鼠经历母婴分离会显著改变嗅觉神经发生区域——脑室下区（SVZ）和吻侧迁移流（RMS）产后神经发生的各个过程。为了确定母婴分离对产后神经发生的应激影响，我们研究了短期母婴分离引起的嗅觉环境改变是否会诱导新生大鼠SVZ/RMS以及嗅觉皮质区域——前嗅核（AON）中Fos蛋白的表达。在出生后第7、14和21天，将幼崽与母亲分离2小时。对免疫组织化学标记的Fos蛋白进行评估。我们的结果显示，单次母婴分离是一个应激事件，它以年龄依赖性方式选择性地刺激SVZ和AON中的细胞活性。在P21对照动物的SVZ中发现了一些Fos+细胞，母婴分离显著增加了它们的数量。这表明一些SVZ细胞包含在由母婴分离激活的神经回路中，并且这些细胞具备完整的Fos信号转导机制。在所有检测的年龄阶段，母婴分离均显著增加了AON中Fos+细胞的数量，这表明其作用是由嗅觉介导的。
