T抗原核转运缺陷的猿猴病毒40突变体诱导细胞DNA合成
Induction of cellular DNA synthesis by a simian virus 40 mutant defective in nuclear transport of T antigen.
作者信息
Lanford R E, Hyland J K, Baserga R, Butel J S
出版信息
Mol Cell Biol. 1985 Jun;5(6):1531-3. doi: 10.1128/mcb.5.6.1531-1533.1985.
Abstract
The simian virus 40 (SV40) (cT)-3 mutant [SV40(cT)-3], which is defective in nuclear transport of T antigen, was utilized to determine whether cellular DNA synthesis can be stimulated by SV40 in the absence of detectable nuclear T antigen. Cellular DNA synthesis was examined in the temperature-sensitive cell cycle mutants, BHK ts13 and BHK tsAF8, after microinjection of quiescent cells with plasmid DNA containing cloned copies of wild-type SV40 or SV40(cT)-3. The efficiency of induction of cellular DNA synthesis was identical for both wild-type SV40 and SV40(cT)-3 in both cell lines. The results suggest that cell surface-associated T antigen, either alone or possibly in combination with minimal amounts of nuclear T antigen below our limit of detection, is able to stimulate cellular DNA synthesis.
摘要
猿猴病毒40（SV40）（cT）-3突变体[SV40（cT）-3]在T抗原的核转运方面存在缺陷，被用于确定在没有可检测到的核T抗原的情况下，SV40是否能刺激细胞DNA合成。在用含有野生型SV40或SV40（cT）-3克隆拷贝的质粒DNA显微注射静止细胞后，在温度敏感的细胞周期突变体BHK ts13和BHK tsAF8中检测细胞DNA合成。在这两种细胞系中，野生型SV40和SV40（cT）-3诱导细胞DNA合成的效率相同。结果表明，细胞表面相关的T抗原，无论是单独存在，还是可能与低于我们检测限的少量核T抗原结合，都能够刺激细胞DNA合成。
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