Cupido M, Bridges B A
Mutat Res. 1985 Sep;146(2):135-41. doi: 10.1016/0167-8817(85)90003-3.
On the basis of survival data, repair of 8-methoxypsoralen DNA crosslinks in Escherichia coli strains lacking a functional uvrABC endonuclease, is shown to require the products of the recA, recB, recF and recN genes. Bacteria, grown under conditions where most cells contain only a single genome, show no evidence of crosslink repair. Similarly, bacteriophage lambda shows evidence of crosslink repair only in SOS-induced cells, and only at multiplicities of infection greater than 1. The requirement for rec+ genes may be partly ascribed to the need for a functional SOS response, but taken together, the results suggest a recombinational step involving a homologous region of DNA may occur during uvr-independent crosslink repair.
基于生存数据，在缺乏功能性uvrABC核酸内切酶的大肠杆菌菌株中，8-甲氧基补骨脂素DNA交联的修复显示需要recA、recB、recF和recN基因的产物。在大多数细胞仅含有单个基因组的条件下生长的细菌，没有显示交联修复的证据。同样，噬菌体λ仅在SOS诱导的细胞中，且仅在感染复数大于1时显示交联修复的证据。对rec⁺基因的需求可能部分归因于对功能性SOS反应的需要，但综合起来，结果表明在不依赖uvr的交联修复过程中可能会发生涉及DNA同源区域的重组步骤。
