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紫外线照射后recB功能正常的大肠杆菌K12(λ)溶原菌中的原噬菌体失活

Prophage inactivation in recB-proficient Escherichia coli K12 (lambda) lysogens after ultraviolet irradiation.

作者信息

Petranović M, Petranović D, Salaj-Smic E, Trgovcević Z

出版信息

Mol Gen Genet. 1984;196(1):167-9. doi: 10.1007/BF00334110.

DOI:10.1007/BF00334110
PMID:6237245
Abstract

If ultraviolet irradiated, lambda-lysogenic Escherichia coli K12 bacteria are incubated for 4 to 6 h at 30 degrees C, lambda prophage becomes inactive in the non-surviving cells. This was demonstrated by the use of lambda cIts857ind1 prophage which can be induced by heat but not by ultraviolet light. An analysis with various bacterial mutants showed that RecBC recombination enzyme is required in conjunction with RecA protein for prophage inactivation.

摘要

如果用紫外线照射,将λ-溶原性大肠杆菌K12细菌在30℃下培养4至6小时,λ原噬菌体在不能存活的细胞中会变得无活性。这是通过使用λcIts857ind1原噬菌体证明的,该原噬菌体可被热诱导但不能被紫外线诱导。对各种细菌突变体的分析表明,原噬菌体失活需要RecBC重组酶与RecA蛋白协同作用。

相似文献

1
Prophage inactivation in recB-proficient Escherichia coli K12 (lambda) lysogens after ultraviolet irradiation.紫外线照射后recB功能正常的大肠杆菌K12(λ)溶原菌中的原噬菌体失活
Mol Gen Genet. 1984;196(1):167-9. doi: 10.1007/BF00334110.
2
Lysogenization of ultraviolet-irradiated Escherichia coli with lambda: dependence on the recA and recB gene products.用λ噬菌体对紫外线照射的大肠杆菌进行溶原化:对recA和recB基因产物的依赖性。
Nucleic Acids Res. 1984 Feb 10;12(3):1563-72. doi: 10.1093/nar/12.3.1563.
3
Inactivation of prophage in ultraviolet-irradiated Escherichia coli: dependence on recA gene activity.紫外线照射的大肠杆菌中前噬菌体的失活:对recA基因活性的依赖性。
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Kinetics of recB-dependent repair: relationship to post-UV inactivation of the prophage.
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ULTRAVIOLET SENSITIVITY OF ESCHERICHIA COLI CONTAINING HEAT-INDUCIBLE LAMBDA PROPHAGES.含有热诱导性λ原噬菌体的大肠杆菌的紫外线敏感性
Science. 1964 Jul 10;145(3628):175-6. doi: 10.1126/science.145.3628.175.

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本文引用的文献

1
The SOS regulatory system of Escherichia coli.大肠杆菌的SOS调控系统。
Cell. 1982 May;29(1):11-22. doi: 10.1016/0092-8674(82)90085-x.
2
Prophage phi 80 is induced in Escherichia coli K12 recA430.原噬菌体φ80在大肠杆菌K12 recA430中被诱导。
Mol Gen Genet. 1983;189(2):199-206. doi: 10.1007/BF00337804.
3
A fine structure map of spontaneous and induced mutations in the lambda repressor gene, including insertions of IS elements.λ阻遏蛋白基因中自发突变和诱导突变的精细结构图谱,包括IS元件的插入。
Mol Gen Genet. 1981;184(3):364-71. doi: 10.1007/BF00352506.
4
Lysogenization of ultraviolet-irradiated Escherichia coli with lambda: dependence on the recA and recB gene products.用λ噬菌体对紫外线照射的大肠杆菌进行溶原化:对recA和recB基因产物的依赖性。
Nucleic Acids Res. 1984 Feb 10;12(3):1563-72. doi: 10.1093/nar/12.3.1563.
5
Isolation and genetic analysis of a strain of Escherichia coli K-12 with an amber recA mutation.一株具有琥珀型recA突变的大肠杆菌K-12菌株的分离与遗传分析。
J Bacteriol. 1971 Jul;107(1):388-9. doi: 10.1128/jb.107.1.388-389.1971.
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Genetic recombination in Escherichia coli: the role of exonuclease I.大肠杆菌中的基因重组:核酸外切酶I的作用。
Proc Natl Acad Sci U S A. 1971 Apr;68(4):824-7. doi: 10.1073/pnas.68.4.824.
7
Pedigrees of some mutant strains of Escherichia coli K-12.大肠杆菌K-12某些突变菌株的谱系。
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Dominant mutations (lex) in Escherichia coli K-12 which affect radiation sensitivity and frequency of ultraviolet lght-induced mutations.大肠杆菌K-12中影响辐射敏感性和紫外线诱导突变频率的显性突变(lex)
J Bacteriol. 1972 Nov;112(2):886-93. doi: 10.1128/jb.112.2.886-893.1972.
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Biochemical and genetic studies of recombination proficiency in Escherichia coli. II. Rec+ revertants caused by indirect suppression of rec- mutations.大肠杆菌中重组能力的生化与遗传学研究。II. 由rec-突变的间接抑制导致的Rec+回复突变体
Proc Natl Acad Sci U S A. 1970 Sep;67(1):128-35. doi: 10.1073/pnas.67.1.128.
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Recombination deficient mutants of E. coli and other bacteria.大肠杆菌及其他细菌的重组缺陷型突变体。
Annu Rev Genet. 1973;7:67-86. doi: 10.1146/annurev.ge.07.120173.000435.