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紫外线照射后recB功能正常的大肠杆菌K12(λ)溶原菌中的原噬菌体失活

Prophage inactivation in recB-proficient Escherichia coli K12 (lambda) lysogens after ultraviolet irradiation.

作者信息

Petranović M, Petranović D, Salaj-Smic E, Trgovcević Z

出版信息

Mol Gen Genet. 1984;196(1):167-9. doi: 10.1007/BF00334110.

Abstract

If ultraviolet irradiated, lambda-lysogenic Escherichia coli K12 bacteria are incubated for 4 to 6 h at 30 degrees C, lambda prophage becomes inactive in the non-surviving cells. This was demonstrated by the use of lambda cIts857ind1 prophage which can be induced by heat but not by ultraviolet light. An analysis with various bacterial mutants showed that RecBC recombination enzyme is required in conjunction with RecA protein for prophage inactivation.

摘要

如果用紫外线照射,将λ-溶原性大肠杆菌K12细菌在30℃下培养4至6小时,λ原噬菌体在不能存活的细胞中会变得无活性。这是通过使用λcIts857ind1原噬菌体证明的,该原噬菌体可被热诱导但不能被紫外线诱导。对各种细菌突变体的分析表明,原噬菌体失活需要RecBC重组酶与RecA蛋白协同作用。

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