SOX-2 在口腔白斑病中的免疫表达。
Immunoexpression of SOX-2 in oral leukoplakia.
机构信息
Pontifícia Universidade Católica do Paraná, Curitiba, Brasil.
Universidade Federal de Santa Catarina, Florianópolis, Brasil.
出版信息
Oral Dis. 2018 Nov;24(8):1449-1457. doi: 10.1111/odi.12922. Epub 2018 Jul 18.
OBJECTIVE
This study was conducted to correlate and compare the immunoexpression of sex-determining region Y-box 2 (SOX-2) in oral leukoplakia (OL) lesions with that in normal buccal mucosa (control).
MATERIALS AND METHODS
In this observational study, OL with low-risk (n = 34) and high-risk (n = 33) dysplasia and control samples (n = 25) were subjected to immunohistochemical analysis for SOX-2. In the epithelium, SOX-2 positive and negative cells, as well as semiautomatic segmentation of the immunopositive nuclear area were counted. Statistical tests included chi-square, one-way analysis of variance, Tukey, and Games-Howell. The level of significance was 5%.
RESULTS
Groups with OL lesions (low and high-risk) showed higher mean numbers of SOX-2 positive cells (63.47 ± 25.70 and 68.18 ± 21.17) compared to the control group (45.85 ± 27.38) (p = 0.00). Groups with OL lesions (low and high-risk) exhibited higher mean positive nuclear area (0.24 ± 0.47 and 1.09 ± 2.06) compared to the control group (0.00 ± 0.01) (p = 0.01).
CONCLUSION
Oral leukoplakia lesions showed a higher expression of SOX-2, suggesting its contribution to the pathogenesis of OL.
目的
本研究旨在比较和分析性别决定区 Y 框 2（SOX-2）在口腔白斑（OL）病变中的免疫表达与正常颊黏膜（对照）中的表达。
材料与方法
在这项观察性研究中，对低风险（n=34）和高风险（n=33）异型增生的 OL 病变以及对照样本（n=25）进行 SOX-2 的免疫组织化学分析。在上皮中，对 SOX-2 阳性和阴性细胞以及免疫阳性核区的半自动分割进行计数。统计检验包括卡方检验、单因素方差分析、Tukey 和 Games-Howell。显著性水平为 5%。
结果
OL 病变组（低风险和高风险）的 SOX-2 阳性细胞平均数量（63.47±25.70 和 68.18±21.17）高于对照组（45.85±27.38）（p=0.00）。OL 病变组（低风险和高风险）的阳性核区平均面积（0.24±0.47 和 1.09±2.06）高于对照组（0.00±0.01）（p=0.01）。
结论
口腔白斑病变中 SOX-2 的表达较高，提示其对 OL 的发病机制有一定贡献。
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