Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India.
Asian Pac J Cancer Prev. 2020 Nov 1;21(11):3317-3323. doi: 10.31557/APJCP.2020.21.11.3317.
Stathmin is an intracellular phosphoprotein that controls the microtubule dynamics by further regulating proper attachment and alignment of chromosomes in a dividing cell. Thus, any mutation or aberrantly expressed protein that reduces the fidelity of spindle assembly will enhance chromosomal instability contributing to aneuploidy. Oral Squamous Cell Carcinoma is an extensively studied malignancy that occurs due to accumulated genetic changes due to carcinogens. The current study is done to evaluate the stathmin role and its expression in OSCC and Oral epithelial dysplasia (OED).
The aim of the present study is to evaluate the role of stathmin in OSCC and Oral dysplasia and also to correlate the expression of Stathmin with respect to the different histopathological grades of OED and OSCC.
30 neutral buffered formalin fixed, paraffin embedded (FFPE) tissues of Oral Leukoplakia/OED and 30 FFPE tissues of OSCC were subjected to immunohistochemistry with stathmin antibody. Five fields of each case with 300 cells were examined and a mean percentage of positive-stained slides were determined. The percentages were recorded accordingly with their respective histological grades. The results were analysed statistically.
The results of the present study demonstrated higher mean values of stathmin in tissues with OSCC (2.50) compared to leukoplakia (2.11) and normal tissues (0.00) with a high level of statistical significance (0.0001). There is also an increase in the percentage levels of stathmin with increase in the histological grade of differentiation in OSCC as well as leukoplakia.
The present study found a statistical correlation between increased grades of the disease with expression levels of stathmin. This confirms that stathmin expression can contribute to disease progression and that stathmin might have a potential role as an early diagnostic biomarker and can be a therapeutic target for OSCC.
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Stathmin 是一种细胞内磷酸蛋白，通过进一步调节分裂细胞中染色体的正确附着和排列来控制微管动力学。因此，任何降低纺锤体组装保真度的突变或异常表达的蛋白质都会增强染色体不稳定性，导致非整倍体。口腔鳞状细胞癌是一种广泛研究的恶性肿瘤，由于致癌物引起的遗传变化而发生。本研究旨在评估 Stathmin 在口腔鳞状细胞癌和口腔上皮异型增生（OED）中的作用及其表达。
本研究旨在评估 Stathmin 在口腔鳞状细胞癌和口腔异型增生中的作用，并分析 Stathmin 的表达与 OED 和口腔鳞状细胞癌不同组织病理学分级的关系。
30 例口腔白斑/异型增生（OED）的中性缓冲福尔马林固定、石蜡包埋（FFPE）组织和 30 例口腔鳞状细胞癌的 FFPE 组织均用 Stathmin 抗体进行免疫组织化学染色。每个病例的 5 个视野用 300 个细胞进行检查，并确定阳性染色切片的平均百分比。根据各自的组织学分级记录百分比。结果进行了统计学分析。
本研究结果表明，口腔鳞状细胞癌组织中 Stathmin 的平均数值（2.50）高于口腔白斑（2.11）和正常组织（0.00），具有统计学显著性（0.0001）。在口腔鳞状细胞癌和口腔白斑中，随着组织学分化程度的增加，Stathmin 的百分比水平也有所增加。
本研究发现，疾病分级的增加与 Stathmin 表达水平之间存在统计学相关性。这证实了 Stathmin 的表达可能有助于疾病进展，并且 Stathmin 可能具有作为早期诊断生物标志物的潜力，并可能成为口腔鳞状细胞癌的治疗靶点。
