Mariel Murga Humberto, Centeno Sanchez Rene, Sánchez Meraz Wulfrano, González Amaro Ana María, Arredondo Hérnandez Raymundo, Mariel Cárdenas Jairo, Javier Gutiérrez Cantu Francisco
Invest Clin. 2016 Dec;57(4):321-9.
The antimicrobial efficacy of the silver nanoparticles (NPsAg), incorporated into the adhesive (primer) placed in the enamel adjacent to fixed orthodontic appliances (brackets), was evaluated. An experimental study was performed on 40 premolars in vitro, which were divided into two groups with brackets, one cemented with conventional primer and another added with NPsAg, placed in culture media previously inoculated with Streptococcus mutans, and sampled for culturing and counting colony forming units (UFC) on days 1, 15 and 30. A decrease in the presence of Streptococcus mutans in the samples after 15 days with nanoparticle aggregation was observed, and a reduction in the effect of said nanoparticles after 30 days. This reduction of the nanoparticles effects can be due to the absence of mechanical cleaning, which favored the bacterial aggregation on the biofilm, affecting its antimicrobial effect. This suggest the need for realizing studies in “vivo” which will allow the observation of the behavior of the biometals on the buccal medium. The NPsAg added to the primer are an effective tool to prevent the demineralization of the enamel around the fixed orthodontic appliances.
评估了掺入放置在固定正畸矫治器（托槽）相邻牙釉质中的粘合剂（底漆）中的银纳米颗粒（NPsAg）的抗菌效果。对40颗前磨牙进行了体外实验研究，将其分为两组，每组都带有托槽，一组用传统底漆粘结，另一组添加了NPsAg，置于预先接种变形链球菌的培养基中，并在第1天、第15天和第30天取样进行培养和计数菌落形成单位（UFC）。观察到15天后纳米颗粒聚集，样品中变形链球菌数量减少，30天后所述纳米颗粒的效果降低。纳米颗粒效果的这种降低可能是由于缺乏机械清洁，这有利于细菌在生物膜上聚集，影响其抗菌效果。这表明需要进行“体内”研究，以便观察生物金属在口腔介质中的行为。添加到底漆中的NPsAg是预防固定正畸矫治器周围牙釉质脱矿的有效工具。
