Amano S, Sasahara M, Yamada E, Kataoka H, Hazama F
Neuropathol Appl Neurobiol. 1985 May-Jun;11(3):161-70. doi: 10.1111/j.1365-2990.1985.tb00014.x.
Changes of 5'-nucleotidase (5'-N) activity during subcultivation of C6 clonal rat glioma cells were studied histochemically, cytochemically and biochemically. Two different cell populations were seeded with high or low cell density respectively. 5'-N activity in cultured cells increased continuously in both populations. The enzyme activity was always higher in the cells growing in high density than in those growing in low density, and this difference became statistically significant after 6 days of subcultivation. It seemed likely that the increase in 5'-N activity depended on cell density, not on the frequency of mitosis. Cytochemically, enzyme activity was detected on the outer surface of the plasma membrane of cell bodies or cell processes. Strong activity was found on the processes and cell membranes where cells touched each other. In the regions where cells had intimate cell-to-cell contact, marked elevation of the enzyme activity was seen. Increased 5'-N activity, which appeared to depend on cell density, was considered to be related with this cell-to-cell contact.
采用组织化学、细胞化学和生物化学方法研究了C6克隆大鼠胶质瘤细胞传代培养过程中5'-核苷酸酶（5'-N）活性的变化。分别以高细胞密度或低细胞密度接种两种不同的细胞群体。两种群体中培养细胞的5'-N活性均持续增加。高密度生长的细胞中的酶活性始终高于低密度生长的细胞，传代培养6天后这种差异具有统计学意义。5'-N活性的增加似乎取决于细胞密度，而非有丝分裂频率。细胞化学检测显示，酶活性存在于细胞体或细胞突起的质膜外表面。在细胞相互接触的突起和细胞膜上发现强活性。在细胞间紧密接触的区域，可见酶活性显著升高。5'-N活性的增加似乎取决于细胞密度，被认为与这种细胞间接触有关。
