Asou H, Mutou N, Hirano S
Neurosci Res. 1985 Jun;2(5):399-406. doi: 10.1016/0168-0102(85)90050-1.
The distribution of ganglioside GM1 in primary cultured cells from rat cerebral hemispheres was studied. These dissociated cell cultures are capable of forming myelin. 2',3'-Cyclic nucleotide 3'-phosphodiesterase (CNPase) activity, which is considered to be a good marker for myelin, increased with the onset of myelin formation. Ganglioside GM1 was localized on the myelin-related structures using a biotinylated choleragen and avidin peroxidase technique. The distribution of ganglioside GM1 resembled that of immunostained CNPase.
研究了神经节苷脂GM1在大鼠大脑半球原代培养细胞中的分布。这些解离细胞培养物能够形成髓磷脂。2',3'-环核苷酸3'-磷酸二酯酶（CNPase）活性被认为是髓磷脂的良好标志物，它随着髓磷脂形成的开始而增加。使用生物素化霍乱毒素和抗生物素蛋白过氧化物酶技术将神经节苷脂GM1定位在与髓磷脂相关的结构上。神经节苷脂GM1的分布与免疫染色的CNPase相似。
