Hirano S, Iwasaki-Mutou N, Asou H, Ogawa Y
Cell Struct Funct. 1985 Sep;10(3):271-8. doi: 10.1247/csf.10.271.
Developing cultures of dissociated cerebral hemispheres obtained from 18-day-old embryonic rats synthesized, or activated, a myelin-related enzyme, 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNPase). This increase in activity coincided with the onset of myelination. The presence of CNPase in oligodendrocytes and myelin was demonstrated using immunocytochemical staining techniques. Myelinated axons and myelin sheaths were clearly made visible by electron microscopy of 28-day-old cultures.
从18日龄胚胎大鼠获得的解离大脑半球培养物合成或激活了一种髓鞘相关酶，即2',3'-环核苷酸3'-磷酸水解酶（CNPase）。这种活性增加与髓鞘形成的开始相一致。使用免疫细胞化学染色技术证实了少突胶质细胞和髓鞘中存在CNPase。通过对28日龄培养物进行电子显微镜观察，清晰可见有髓轴突和髓鞘。
