State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Dermatitis. 2018 Jul/Aug;29(4):219-222. doi: 10.1097/DER.0000000000000392.
Atopic dermatitis (AD) is a common disease, which involves a disruption of the skin barrier function. Skin ceramide (CER) composition, which plays crucial roles in maintaining the barrier function of the stratum corneum, is changed in patients with AD.
The aim of this study was to identify and quantify skin CER subclasses in association with disease severity in pediatric patients with AD.
Two hundred thirteen patients were entered into the observational study. We compared their CER profiles using normal-phase high-performance liquid chromatography coupled with dynamic multiple reaction monitoring mass spectrometry.
In total, 12 subclasses of CERs were identified. We found that 2 subclasses, that is, CER[AS] and CER[NS], were elevated (P = 0.007 and 0.012, respectively) and correlated with Severity Scoring of Atopic Dermatitis (P = 0.004 and 0.004, respectively).
Skin CER abundances are changed in children with AD compared with control subjects.
特应性皮炎（AD）是一种常见疾病，其涉及皮肤屏障功能障碍。皮肤神经酰胺（CER）的组成在维持角质层的屏障功能方面起着至关重要的作用，在 AD 患者中发生改变。
本研究旨在确定并定量与疾病严重程度相关的患有 AD 的儿科患者的皮肤 CER 亚类。
将 213 名患者纳入观察性研究。我们使用正相高效液相色谱法与动态多重反应监测质谱法比较了他们的 CER 谱。
总共鉴定出 12 种 CER 亚类。我们发现 2 种亚类，即 CER[AS]和 CER[NS]升高（P = 0.007 和 0.012），并且与特应性皮炎严重程度评分相关（P = 0.004 和 0.004）。
与对照组相比，患有 AD 的儿童的皮肤 CER 丰度发生了变化。
