从人红细胞中大规模纯化泛素。
The large scale purification of ubiquitin from human erythrocytes.
作者信息
Haas A L, Wilkinson K D
出版信息
Prep Biochem. 1985;15(1-2):49-60. doi: 10.1080/00327488508062433.
PMID:2994034
Abstract
A simple, reproducible method for the large-scale purification of active ubiquitin from human erythrocytes is described. Erythrocytes contain 100 micrograms free ubiquitin per cc of packed cells, of which 44% can be recovered in homogeneous form by a combination of heat treatment, ammonium sulfate fractionation, and ion exchange chromatography.
摘要
本文描述了一种从人红细胞中大规模纯化活性泛素的简单、可重复的方法。每立方厘米压实红细胞中含有100微克游离泛素，其中44%可通过热处理、硫酸铵分级分离和离子交换色谱法相结合以均一形式回收。
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