乙酰胆碱和佛波酯可抑制非洲爪蟾卵母细胞中由腺苷和环磷酸腺苷诱发的钾电流。
Acetylcholine and phorbol esters inhibit potassium currents evoked by adenosine and cAMP in Xenopus oocytes.
作者信息
Dascal N, Lotan I, Gillo B, Lester H A, Lass Y
出版信息
Proc Natl Acad Sci U S A. 1985 Sep;82(17):6001-5. doi: 10.1073/pnas.82.17.6001.
Abstract
In Xenopus laevis oocytes, adenosine and other purinergic agonists induce a K+-conductance increase that is fully mimicked by intracellular application of cAMP. Acetylcholine suppresses the K+-conductance increase caused by adenosine, by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, or by intracellular injection of cAMP. This effect of acetylcholine is not mimicked by intracellular injection of Ca2+ or of the Ca-mobilizing agent inositol 1,4,5-trisphosphate. However, adenosine and cAMP responses are inhibited by 4 beta-phorbol 12,13-dibutyrate and 4 beta-phorbol 12-myristate 13-acetate. These results suggest that, in Xenopus oocytes, the muscarinic inhibition of purinergic and cAMP responses is mediated through the activation of the phospholipid-dependent, Ca-activated protein kinase (protein kinase C).
摘要
在非洲爪蟾卵母细胞中，腺苷及其他嘌呤能激动剂可诱导钾离子电导增加，而细胞内施加环磷酸腺苷（cAMP）可完全模拟这种增加。乙酰胆碱可抑制由腺苷、磷酸二酯酶抑制剂3 -异丁基- 1 -甲基黄嘌呤或细胞内注射cAMP所引起的钾离子电导增加。乙酰胆碱的这种作用不能通过细胞内注射钙离子或钙动员剂肌醇1,4,5 -三磷酸来模拟。然而，腺苷和cAMP反应可被4β -佛波醇12,13 -二丁酸酯和4β -佛波醇12 -肉豆蔻酸酯13 -乙酸酯抑制。这些结果表明，在非洲爪蟾卵母细胞中，毒蕈碱对嘌呤能和cAMP反应的抑制是通过磷脂依赖性、钙激活蛋白激酶（蛋白激酶C）的激活介导的。
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本文引用的文献
1
Types of muscarinic response in Xenopus oocytes.非洲爪蟾卵母细胞中的毒蕈碱反应类型。
Life Sci. 1980 Oct 13;27(15):1423-8. doi: 10.1016/0024-3205(80)90407-5.
2
Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.非洲爪蟾卵母细胞膜中的胆碱能和儿茶酚胺能受体。
J Physiol. 1982 Jul;328:143-70. doi: 10.1113/jphysiol.1982.sp014257.
3
Role of cyclic nucleotides in excitable cells.环核苷酸在可兴奋细胞中的作用。
Annu Rev Physiol. 1980;42:629-41. doi: 10.1146/annurev.ph.42.030180.003213.
4
5
Influence of a tumor-promoting phorbol ester on the electrical response of B-cells to glucose and glyburide.一种促肿瘤佛波酯对B细胞对葡萄糖和格列本脲电反应的影响。
Mol Pharmacol. 1984 Sep;26(2):267-71.
6
Time course of the increase in the myocardial slow inward current after a photochemically generated concentration jump of intracellular cAMP.细胞内cAMP光化学诱导浓度跃升后心肌慢内向电流增加的时间进程。
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2395-9. doi: 10.1073/pnas.80.8.2395.
7
Molecular biology of learning: modulation of transmitter release.学习的分子生物学：神经递质释放的调节
Science. 1982 Oct 29;218(4571):433-43. doi: 10.1126/science.6289442.
8
Adenosine-induced slow ionic currents in the Xenopus oocyte.非洲爪蟾卵母细胞中腺苷诱导的慢离子电流。
Nature. 1982 Aug 5;298(5874):572-4. doi: 10.1038/298572a0.
9
Characterization of the soluble cyclic nucleotide phosphodiesterases in Xenopus laevis oocytes. Evidence for a calmodulin-dependent enzyme.非洲爪蟾卵母细胞中可溶性环核苷酸磷酸二酯酶的特性。一种钙调蛋白依赖性酶的证据。
Biochim Biophys Acta. 1982 Feb 18;701(2):253-9. doi: 10.1016/0167-4838(82)90121-2.
10
The molecular basis of neurotransmission at the muscarinic receptor.
Biochem Pharmacol. 1981 Nov 1;30(21):2887-91. doi: 10.1016/0006-2952(81)90248-3.
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