Hematology Department, Shengjing Hospital, China Medical University, Shenyang, Liaoning Province, China.
Neoplasma. 2018 Sep 19;65(5):807-814. doi: 10.4149/neo_2018_171028N691. Epub 2018 Jun 17.
The relationships between autophagy-associated gene expression and clinical characteristics and prognosis in acute myeloid leukemia (AML) have not been well revealed. We examined mRNA expression of Bcl-2, p62, Beclin 1, VPS34, Rubicon, ALFY, UVRAG, ULK1, LC3 and NBR1 in 20 AML cases and 10 benign hematological cases by real-time PCR. Clinical information, treatment responses and outcomes of the AML patients were collected. Beclin 1, LC3, UVRAG, Rubicon and NBR1 were downregulated in AML patients compared with control group (P<0.05). Low ULK1 expression was associated with high white blood cell counts (P<0.05). Autophagy-associated gene expression was not correlated with chemotherapy response. Finally, we analyzed overall survival and found no obvious association with gene expression. However, in unfavorable outcome patients, low Beclin 1 and p62 expression showed worse overall survival than high-expression. Autophagy genes are associated with outcome in AML patients and may be biomarkers or targets in the future.
自噬相关基因表达与急性髓系白血病（AML）的临床特征和预后之间的关系尚未得到充分揭示。我们通过实时 PCR 检测了 20 例 AML 病例和 10 例良性血液病例中 Bcl-2、p62、Beclin 1、VPS34、Rubicon、ALFY、UVRAG、ULK1、LC3 和 NBR1 的 mRNA 表达。收集了 AML 患者的临床信息、治疗反应和结局。与对照组相比，AML 患者中 Beclin 1、LC3、UVRAG、Rubicon 和 NBR1 下调（P<0.05）。低 ULK1 表达与高白细胞计数相关（P<0.05）。自噬相关基因表达与化疗反应无关。最后，我们分析了总生存率，发现与基因表达没有明显关联。然而，在预后不良的患者中，低 Beclin 1 和 p62 表达的总生存率比高表达差。自噬基因与 AML 患者的结局相关，未来可能成为生物标志物或治疗靶点。
