R & D Center, Cosmocos Corporation, Incheon, 21698, Republic of Korea.
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
BMC Complement Altern Med. 2018 Jun 26;18(1):196. doi: 10.1186/s12906-018-2194-9.
Agastache rugosa (Fisch. & C.A.Mey.) Kuntze (Korean mint) is used to treat diverse types of human disorders in traditional medicine. In recent years, its non-fermented leaf extract (ARE) has been shown to possess protective properties against ultraviolet-B (UV-B) radiation-induced photooxidative stress. The present work aimed to examine whether probiotic bacterial fermentation would potentiate the skin anti-photoaging activity of ARE or not, by comparing the protective properties of ARE and corresponding fermented extract (ARE-F) against UV-B radiation-induced photooxidative stress in HaCaT keratinocytes.
ARE-F was produced from ARE by the fermentation with Lactobacillus rhamnosus HK-9, a type of Gram-positive probiotic bacterial strain. Anti-photoaging activities were evaluated by analyzing reactive oxygen species (ROS), promatrix metalloproteinases (proMMPs), total glutathione (GSH) and total superoxide dismutase (SOD) in UV-B-irradiated HaCaT keratinocytes. Antiradical activity was determined using 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay.
ARE-F contained higher attenuating activity on the UV-B-induced ROS generation than ARE. Similarly, ARE-F was able to diminish the UV-B-induced proMMP-9 and -2 more effectively than ARE. ARE-F displayed higher tendencies to augment the UV-B-reduced total GSH content and SOD activity than ARE. However, there were no significant difference between ARE and ARE-F in ABTS radical scavenging activities.
The findings suggest that the UV-B radiation-protective activity of ARE is enhanced by probiotic bacterial fermentation, which might improve the therapeutic and cosmetic values of A. rugosa leaves.
Agastache rugosa（Fisch. & C.A.Mey.）Kuntze（韩国薄荷）在传统医学中被用于治疗多种人类疾病。近年来，其未发酵的叶提取物（ARE）已被证明具有抵抗紫外线-B（UV-B）辐射诱导的光氧化应激的保护特性。本研究旨在通过比较 ARE 和相应发酵提取物（ARE-F）对 UV-B 辐射诱导的 HaCaT 角质形成细胞光氧化应激的保护特性，来检验益生菌发酵是否会增强 ARE 的皮肤抗光老化活性。
ARE-F 是通过将 ARE 与革兰氏阳性益生菌菌株 Lactobacillus rhamnosus HK-9 发酵制成的。通过分析 UV-B 照射的 HaCaT 角质形成细胞中的活性氧（ROS）、前基质金属蛋白酶（proMMPs）、总谷胱甘肽（GSH）和总超氧化物歧化酶（SOD）来评估抗光老化活性。使用 2,2-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除测定法测定抗自由基活性。
ARE-F 对 UV-B 诱导的 ROS 生成的抑制活性高于 ARE。同样，ARE-F 也能比 ARE 更有效地减少 UV-B 诱导的 proMMP-9 和 -2。ARE-F 提高 UV-B 降低的总 GSH 含量和 SOD 活性的趋势高于 ARE。然而，ARE 和 ARE-F 在 ABTS 自由基清除活性方面没有显著差异。
研究结果表明，益生菌发酵增强了 ARE 的 UV-B 辐射保护活性，这可能提高了 A. rugosa 叶片的治疗和美容价值。
