Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Minhang Branch, Zhongshan Hospital and Institute of Biomedical Sciences, Fudan University, Shanghai, China; Institute of Fudan-Minhang Academic Health System, Minhang Hospital, Fudan University, Shanghai, China.
Adv Cancer Res. 2018;139:185-207. doi: 10.1016/bs.acr.2018.04.006. Epub 2018 May 23.
Cancer immunotherapy fights against cancer by modulating the immune response and is delivering encouraging results in clinical treatments. However, it is challenging to achieve durable response in all cancer patients during treatment due to the diversity and dynamic nature of immune system as well as inter- and intratumor heterogeneity. A comprehensive assessment of system immunity and tumor microenvironment is crucial for effective and safe cancer therapy, which can potentially be resolved by single-cell proteomic analysis. Single-cell proteomic technologies enable system-wide profiling of protein levels in a number of single cells within the immune system and tumor microenvironment, and thereby provide direct assessment of the functional state of the immune cells and tumor-immune interaction that could be used to evaluate efficacy of immunotherapy and to improve clinical outcome. In this chapter, we summarized current single-cell proteomic technologies and their applications in cancer immunotherapy.
癌症免疫疗法通过调节免疫反应来对抗癌症，并在临床治疗中取得了令人鼓舞的效果。然而，由于免疫系统的多样性和动态性以及肿瘤内和肿瘤间的异质性，在治疗过程中要使所有癌症患者都能获得持久的反应是具有挑战性的。全面评估系统免疫和肿瘤微环境对于有效的癌症治疗至关重要，而单细胞蛋白质组学分析有可能解决这个问题。单细胞蛋白质组学技术能够对免疫系统和肿瘤微环境中的多个单细胞中的蛋白质水平进行系统分析，从而直接评估免疫细胞的功能状态和肿瘤免疫相互作用，可用于评估免疫疗法的疗效并改善临床结果。在本章中，我们总结了目前的单细胞蛋白质组学技术及其在癌症免疫疗法中的应用。
