一种用于检测丁型肝炎病毒RNA的新型双靶点实时逆转录聚合酶链反应检测方法。
A new dual-targeting real-time RT-PCR assay for hepatitis D virus RNA detection.
作者信息
Wang Yan, Glenn Jeffrey S, Winters Mark A, Shen Li-Ping, Choong Ingrid, Shi Ya-Lun, Bi Sheng-Li, Ma Li-Ying, Zeng Hui, Zhang Fu-Jie
机构信息
State Key Laboratory of Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing, P.R. China; Clinical and Research Center of Infectious Diseases, Beijing Ditan Hospital, Clinical Center for HIV/AIDS, Capital Medical University, Beijing, P.R. China.
Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.
出版信息
Diagn Microbiol Infect Dis. 2018 Oct;92(2):112-117. doi: 10.1016/j.diagmicrobio.2018.05.016. Epub 2018 May 29.
In this study, a real-time reverse transcription-polymerase chain reaction (real time RT-PCR) assay targeting 2 genetic segments was established to detect HDV RNA. Utilizing the World Health Organization International Standard for Hepatitis D Virus RNA, the lower limit of detection was 575 IU/mL, and the linearity of quantification ranged from 575,000 IU/mL to 575 IU/mL. 384 HBsAg-positive samples collected from China were tested by this method and HDV antibody detection. Eleven samples were positive for anti-HDV IgG which may persist after HDV resolution, 6 samples were HDV RNA positive, and 5 samples were positive for anti-HDV IgM. This assay showed more sensitivity than the detection of anti-HDV IgM. These data demonstrate that the real-time RT-PCR assay for HDV RNA could be implemented in the clinical detection of HDV infection in chronic HBV-infected patients in China.
在本研究中，建立了一种针对2个基因片段的实时逆转录-聚合酶链反应（实时RT-PCR）检测方法来检测丁型肝炎病毒（HDV）RNA。利用世界卫生组织丁型肝炎病毒RNA国际标准品，检测下限为575 IU/mL，定量线性范围为575,000 IU/mL至575 IU/mL。采用该方法和HDV抗体检测对收集自中国的384份HBsAg阳性样本进行检测。11份样本抗-HDV IgG阳性，其可能在HDV清除后持续存在；6份样本HDV RNA阳性；5份样本抗-HDV IgM阳性。该检测方法显示出比抗-HDV IgM检测更高的灵敏度。这些数据表明，HDV RNA实时RT-PCR检测方法可用于中国慢性HBV感染患者HDV感染的临床检测。
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