从人类X染色体远端分离随机X序列并进行区域定位。
Isolation and regional mapping of random X sequences from distal human X chromosome.
作者信息
Murphy P D, Ruddle F H
出版信息
Somat Cell Mol Genet. 1985 Sep;11(5):433-44. doi: 10.1007/BF01534837.
Chromosome-mediated gene transfer (CMGT) lines were shown to be convenient donors of genomic sequences from specific regions of the genome adjacent to selectable markers. Two libraries were prepared from CMGT lines carrying sequences spanning the long arm of the human X chromosome from HPRT (Xq26) to G6PD (Xq28). A series of 22 CMGT lines sharing the same selectable marker (HPRT) were used in conjunction with five standard translocation hybrids to provide fine-resolution regional mapping of the nonrepetitive X specific probes isolated from the libraries. The order of three human recombinant sequences with respect to known X-linked markers is: PGK (Xq13), 05-02 (DXS78); HPRT (Xq26), 07-03 (DXS79); surface antigen S11 (Xq27), 07-14 (DXS80); and G6PD (Xq28).
染色体介导的基因转移（CMGT）系被证明是来自与选择标记相邻的基因组特定区域的基因组序列的便利供体。从携带跨越人类X染色体长臂从次黄嘌呤磷酸核糖转移酶（HPRT，Xq26）到葡萄糖-6-磷酸脱氢酶（G6PD，Xq28）序列的CMGT系制备了两个文库。一系列22个共享相同选择标记（HPRT）的CMGT系与五个标准易位杂种一起使用，以对从文库中分离出的非重复X特异性探针进行高分辨率区域定位。三个人类重组序列相对于已知X连锁标记的顺序是：磷酸甘油酸激酶（PGK，Xq13），05 - 02（DXS78）；次黄嘌呤磷酸核糖转移酶（HPRT，Xq26），07 - 03（DXS79）；表面抗原S11（Xq27），07 - 14（DXS80）；以及葡萄糖-6-磷酸脱氢酶（G6PD，Xq28）。
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