Müller U, Tantravahi U, Monaco A, Stroh H, Kunkel L M, Latt S A
Hum Genet. 1986 Sep;74(1):24-9. doi: 10.1007/BF00278780.
Two DNA probes from within a single large insert from a recombinant phage-DNA library that was constructed from flow-sorted chromosomes enriched for the human X chromosome were shown to hybridize with repeated X-specific and autosomal DNA sequences. The X-chromosomal repeated sequences were assigned to the distal long arm of the X chromosome by both hybrid mapping and in situ hybridization. Fine mapping places these repeats in a region of Xq28 between DX13 (DXS15, in distal Xq28) and factor VIII (F8C, in proximal Xq28). The location of the X-specific repeats makes them potentially useful for future investigations of diseases mapping to the distal long arm of the X chromosome, such as the fragile X syndrome.
从一个重组噬菌体 - DNA 文库的单个大插入片段中提取的两个 DNA 探针，该文库由富含人类 X 染色体的流式分选染色体构建而成，已显示它们能与重复的 X 特异性和常染色体 DNA 序列杂交。通过杂交定位和原位杂交，将 X 染色体重复序列定位到 X 染色体的远端长臂。精细定位将这些重复序列置于 Xq28 区域中 DX13（远端 Xq28 中的 DXS15）和因子 VIII（近端 Xq28 中的 F8C）之间。X 特异性重复序列的位置使其可能对未来定位到 X 染色体远端长臂的疾病研究有用，比如脆性 X 综合征。
