使用磁分离对顺铂化DNA进行类似酶联免疫吸附测定（ELISA）的分析。

ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation.

作者信息

Smerkova Kristyna, Vlcnovska Marcela, Dostalova Simona, Milosavljevic Vedran, Kopel Pavel, Vaculovic Tomas, Krizkova Sona, Vaculovicova Marketa, Adam Vojtech, Kizek Rene

机构信息

Department of Chemistry and Biochemistry, Laboratory of Metallomics and Nanotechnology, Mendel University in Brno, Brno, Czech Republic.

Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.

出版信息

Nanobiomedicine (Rij). 2015 Jan 1;2:10. doi: 10.5772/61699. eCollection 2015 Jan-Dec.

DOI:10.5772/61699

PMID:29942374

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5997378/

Abstract

Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined advantages of magnetic particles-based isolation/purification with fluorescent properties of quantum dots (QDs) and antibodies targeted on specific recognition of DNA-cisplatin adducts are demonstrated. The formation of a complex between magnetic particles with surface modified by anti-dsDNA antibody, cisplatin-modified DNA and QDs labelled anti-cisplatin-modified DNA antibody was suggested and optimized.

摘要

顺铂是使用最广泛的细胞毒性药物之一。DNA-顺铂加合物的存在测定不仅可以标记富含鸟嘌呤的区域，还可以从相互作用速度方面监测DNA与药物之间的相互作用反应。在这项工作中，展示了基于磁性颗粒的分离/纯化与量子点（QDs）的荧光特性以及靶向特异性识别DNA-顺铂加合物的抗体的综合优势。提出并优化了由抗双链DNA抗体修饰表面的磁性颗粒、顺铂修饰的DNA和标记有抗顺铂修饰DNA抗体的量子点之间形成复合物的过程。