Nejdl Lukas, Kudr Jiri, Cihalova Kristyna, Chudobova Dagmar, Zurek Michal, Zalud Ludek, Kopecny Lukas, Burian Frantisek, Ruttkay-Nedecky Branislav, Krizkova Sona, Konecna Marie, Hynek David, Kopel Pavel, Prasek Jan, Adam Vojtech, Kizek Rene
Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Czech Republic.
Electrophoresis. 2014 Aug;35(16):2333-45. doi: 10.1002/elps.201300576. Epub 2014 Apr 15.
Remote-controlled robotic systems are being used for analysis of various types of analytes in hostile environment including those called extraterrestrial. The aim of our study was to develop a remote-controlled robotic platform (ORPHEUS-HOPE) for bacterial detection. For the platform ORPHEUS-HOPE a 3D printed flow chip was designed and created with a culture chamber with volume 600 μL. The flow rate was optimized to 500 μL/min. The chip was tested primarily for detection of 1-naphthol by differential pulse voltammetry with detection limit (S/N = 3) as 20 nM. Further, the way how to capture bacteria was optimized. To capture bacterial cells (Staphylococcus aureus), maghemite nanoparticles (1 mg/mL) were prepared and modified with collagen, glucose, graphene, gold, hyaluronic acid, and graphene with gold or graphene with glucose (20 mg/mL). The most up to 50% of the bacteria were captured by graphene nanoparticles modified with glucose. The detection limit of the whole assay, which included capturing of bacteria and their detection under remote control operation, was estimated as 30 bacteria per μL.
遥控机器人系统正被用于在包括外星环境在内的恶劣环境中分析各种类型的分析物。我们研究的目的是开发一个用于细菌检测的遥控机器人平台（ORPHEUS-HOPE）。对于ORPHEUS-HOPE平台，设计并制作了一个3D打印的流动芯片，其带有一个体积为600微升的培养室。流速被优化至500微升/分钟。该芯片主要通过差分脉冲伏安法进行1-萘酚检测测试，检测限（信噪比=3）为20纳摩尔。此外，优化了捕获细菌的方法。为了捕获细菌细胞（金黄色葡萄球菌），制备了磁赤铁矿纳米颗粒（1毫克/毫升），并用胶原蛋白、葡萄糖、石墨烯、金、透明质酸以及石墨烯与金或石墨烯与葡萄糖（20毫克/毫升）进行修饰。用葡萄糖修饰的石墨烯纳米颗粒最多可捕获50%的细菌。整个检测的检测限，包括细菌捕获及其在遥控操作下的检测，估计为每微升30个细菌。
