温敏聚合物磁性粒子的制备及其在蛋白质分离中的应用。
Preparation of thermosensitive polymer magnetic particles and their application in protein separations.
机构信息
State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Sichuan University, Chengdu 610065, China.
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
出版信息
J Colloid Interface Sci. 2014 Dec 1;435:99-104. doi: 10.1016/j.jcis.2014.07.007. Epub 2014 Jul 18.
This paper presents a kind of thermoresponsive polymeric magnetic particles for protein separations. The magnetofluids were directly encapsulated in hollow particles constructed by self-assembly of rod-coil poly(ethylene glycol)-poly(N-isopropylacrylamide)/α-cyclodextrin (PEG-PNIPAM/α-CD) complexes. The resulting particles showed reversible protein absorption/desorption capacity because the reversible thermo-sensitivity of PNIPAM. Above the lower critical solution temperature (LCST) of PNIPAM, these particles showed high absorptive capacities and adsorption was done at lower temperature. The protein-laden particles are readily removed from the feed solution in a magnetic field.
本文提出了一种用于蛋白质分离的温敏聚合物磁性粒子。磁流体通过棒-环聚乙二醇-聚（N-异丙基丙烯酰胺）/α-环糊精（PEG-PNIPAM/α-CD）复合物自组装直接包封在空心颗粒中。由于 PNIPAM 的可逆热敏感性，所得粒子表现出可逆的蛋白质吸附/解吸能力。在 PNIPAM 的低临界溶液温度（LCST）以上，这些粒子表现出高的吸附能力，并且在较低温度下进行吸附。负载蛋白质的粒子在磁场中很容易从进料溶液中除去。
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