Shalaby M A, el-Sisi M A, Ismail O E, Afaleque A I
Vet Res Commun. 1985 Jul;9(3):239-244. doi: 10.1007/BF02215148.
A virus was isolated from the brains of pigeons suffering from nervous disorders in different localities of the Eastern Province, Kingdom of Saudi Arabia. The new isolate caused a high morbidity, ranging from 33% to 50%, and a mortality rate which reached 40%. The virus produced pinpoint greyish pock lesions on the chorioallantoic membrane of embryonated hens' eggs and induced syncytial formation followed by rounding and lysis of the cells in chicken embryo fibroblast cultures. Virus infectivity was significantly reduced following treatment by 20% ether or chloroform. The isolated virus was identified as pigeon herpes encephalomyelitis virus by serum-neutralization, agar gel diffusion and fluorescent antibody staining techniques.
从沙特阿拉伯王国东部省不同地区患有神经紊乱的鸽子大脑中分离出一种病毒。这种新分离出的病毒发病率很高，在33%至50%之间，死亡率达40%。该病毒在鸡胚的绒毛尿囊膜上产生针尖大小的灰白色痘疱病变，并在鸡胚成纤维细胞培养物中诱导合胞体形成，随后细胞变圆并裂解。用20%的乙醚或氯仿处理后，病毒感染力显著降低。通过血清中和、琼脂凝胶扩散和荧光抗体染色技术，将分离出的病毒鉴定为鸽疱疹性脑脊髓炎病毒。
