Salem T Z, Zhang F, Sahly N, Thiem S
Biomedical Sciences, University of Science and Technology at Zewail City, October Gardens, 6th of October City, Giza, 12578, Egypt.
Department of Microbial Genetics, AGERI, Agricultural Research Center, Giza, 12619, Egypt.
Mol Biotechnol. 2018 Aug;60(8):576-584. doi: 10.1007/s12033-018-0099-y.
Integral membrane proteins (IMPs) are popular target for drugs, but their resolved structures have been overlooked when compared with cytosolic proteins. The main reason is that IMPs usually need intensive post-translational modifications and they are bound to membranes, which increase the complexity of purifying or crystalizing them. Although different expression systems are used to express IMPs, baculovirus is considered one of the most successful expression systems for those proteins. Despite that, there are always unknown discrepancies in the level of IMPs expression in the baculovirus expression system. Retrospective studies have shown that expression of an immunoglobulin (anti-Chymase mouse monoclonal IgG1) driven by vp39 promoter was more efficient compared to its expression under polyhedrin (polh) promoter; however, this conclusion was not tested on different IMPs to generalize such a conclusion. In this study, the expression of eight different IMPs has been compared under vp39 and polh promoters of Autographa californica nucleopolyhedrovirus. Although different IMPs have shown different patterns of expression, the expression driven by vp39 promoter was found to be generally more efficient than the polh promoter.
整合膜蛋白（IMPs）是药物的热门靶点，但与胞质蛋白相比，其解析结构一直被忽视。主要原因是IMPs通常需要大量的翻译后修饰，并且它们与膜结合，这增加了纯化或结晶它们的复杂性。尽管使用了不同的表达系统来表达IMPs，但杆状病毒被认为是这些蛋白质最成功的表达系统之一。尽管如此，杆状病毒表达系统中IMPs的表达水平总是存在未知差异。回顾性研究表明，由vp39启动子驱动的免疫球蛋白（抗糜酶小鼠单克隆IgG1）的表达比其在多角体蛋白（polh）启动子下的表达更有效；然而，这一结论并未在不同的IMPs上进行测试以推广这一结论。在本研究中，比较了苜蓿银纹夜蛾核多角体病毒的vp39和polh启动子下八种不同IMPs的表达。尽管不同的IMPs表现出不同的表达模式，但发现由vp39启动子驱动的表达通常比polh启动子更有效。
