State Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan, 430072, People's Republic of China.
Mol Genet Genomics. 2018 Dec;293(6):1317-1331. doi: 10.1007/s00438-018-1466-x. Epub 2018 Jun 25.
Pectate lyase catalyses the eliminative cleavage of de-esterified pectin, which is a major component of primary cell walls in many higher plants. Pectate lyase-like (PLL) genes have been identified in various plant species and are involved in a broad range of physiological processes associated with pectin degradation. Previous studies have functionally identified two PLL genes in rice (Oryza sativa. L). However, the knowledge concerning genome-wide analysis of this family remains limited, and functions of the other PLL genes have not been thoroughly elucidated to date. In this study, we identified 12 PLL genes based on a genome-wide investigation in rice. A complete overview of this gene family is presented, including chromosomal locations, exon-intron structure, cis-acting elements and conserved motifs. PLL protein sequences from multiple plant species were compared and divided into five groups based on phylogenetic analysis. Quantitative RT-PCR analysis revealed that only a portion of OsPLL genes (4 of 12) exhibits detectable expression levels. Notably, OsPLL1, OsPLL3, OsPLL4 and OsPLL12 exhibit strong and preferential expression in panicles suggesting that the potential roles of these genes are crucial during rice panicle development. Moreover, knockdown of OsPLL3 and OsPLL4 by artificial microRNA (amiRNA) disrupted normal pollen development and resulted in partial male sterility. These results could provide valuable information for characterising the functions and dissecting the molecular mechanisms of the OsPLL genes.
果胶裂解酶催化去酯化果胶的消除性裂解，果胶是许多高等植物初生细胞壁的主要成分。已在各种植物物种中鉴定出果胶裂解酶样（PLL）基因，并参与与果胶降解相关的广泛的生理过程。先前的研究已经从功能上鉴定了水稻中的两个 PLL 基因。然而，关于该家族的全基因组分析的知识仍然有限，并且迄今为止尚未彻底阐明其他 PLL 基因的功能。在这项研究中，我们根据水稻的全基因组研究鉴定了 12 个 PLL 基因。呈现了该基因家族的完整概述，包括染色体位置、外显子-内含子结构、顺式作用元件和保守基序。比较了来自多种植物物种的 PLL 蛋白序列，并根据系统发育分析将其分为五个组。定量 RT-PCR 分析显示，只有一部分 OsPLL 基因（12 个中的 4 个）表现出可检测的表达水平。值得注意的是，OsPLL1、OsPLL3、OsPLL4 和 OsPLL12 在穗中表现出强烈和优先的表达，表明这些基因的潜在作用在水稻穗发育过程中至关重要。此外，人工 microRNA（amiRNA）敲低 OsPLL3 和 OsPLL4 会破坏正常花粉发育并导致部分雄性不育。这些结果可为表征 OsPLL 基因的功能和剖析其分子机制提供有价值的信息。
