Department of Biology Education, College of Education, Pusan National University, Busan, Korea.
Department of Integrated Omics for Biomedical Sciences, Yonsei University, Seoul, Korea.
J Pharm Pharmacol. 2018 Sep;70(9):1228-1241. doi: 10.1111/jphp.12950. Epub 2018 Jun 25.
To validate the enhanced therapeutic effect of Salvia miltiorrhiza Bunge (SM) for brain ischemic stroke through the anti-apoptotic and survival ability of mesenchymal stem cells (MSCs).
The viability and the expression level of cell apoptotic and survival-related proteins in MSCs by treatment of SM were assessed in vitro. In addition, the infarcted brain region and the behavioural changes after treatment of MSCs with SM were confirmed in rat middle cerebral artery occlusion (MCAo) models.
We demonstrated that SM attenuates apoptosis and improves the cell viability of MSCs. In the rat MCAo model, the recovery of the infarcted region and positive changes of behaviour are observed after treatment of MSCs with SM.
The therapy using SM enhances the therapeutic effect for brain ischemic stroke by promoting the survival of MSCs. This synergetic effect thereby proposes a new experimental approach of traditional Chinese medicine and stem cell-based therapies for patients suffering from a variety of diseases.
通过间充质干细胞（MSCs）的抗凋亡和存活能力，验证丹参对脑缺血性中风的增强治疗效果。
在体外评估丹参处理后 MSCs 的活力和细胞凋亡及存活相关蛋白的表达水平。此外，在大鼠大脑中动脉闭塞（MCAo）模型中证实了丹参处理 MSCs 后梗死脑区和行为变化。
我们表明丹参可减轻 MSCs 的凋亡并提高其细胞活力。在大鼠 MCAo 模型中，用丹参处理 MSCs 后可观察到梗死区的恢复和行为的积极变化。
使用丹参的治疗方法通过促进 MSCs 的存活，增强了对脑缺血性中风的治疗效果。这种协同作用为患有各种疾病的患者提供了一种基于传统中药和干细胞治疗的新实验方法。
