Oh Tae Woo, Park Ki-Ho, Jung Hyo Won, Park Yong-Ki
Department of Herbology, College of Korean Medicine, Dongguk University, Gyeongju, 780-714, South Korea.
Korean Medicine R&D Center, Dongguk University, Gyeongju, 780-714, Republic of Korea.
BMC Complement Altern Med. 2015 Apr 1;15:101. doi: 10.1186/s12906-015-0589-4.
In this study, we investigated the neuroprotective effect of the hairy root extract of Angelica gigas NAKAI (Angelica Gigantis Radix) on transient focal cerebral ischemia in rats through the regulation of angiogenesis molecules.
Male Sprague-Dawley rats were induced focal cerebral ischemia by a transient middle cerebral artery occlusion (tMCAO) for 90 min, and then orally administrated with the water extract of A. gigas hairy roots (AG). After 24 h reperfusion, infarction volume and the changes of BBB permeability were measured by TTC and Evans Blue (EB) staining. The neuronal cell damage and the activation of glial cells were assessed by immunohistochemistry in the ischemic brain. The expression of angiogenesis-induced proteins such as angiopoietin-1 (Ang-1), and vascular endothelial growth factor (VEGF), inflammatory protein such as intercellular adhesion molecule-1 (CAM-1), tight junction proteins such as ZO-1, and Occludin and the phosphorylation of phosphatidylinositol 3-kinase (PI3K)/AKT were determined in the ischemic brains by Western blot, respectively.
The treatment of AG extract significantly decreased the volumes of brain infarction, and edema in MACO-induced ischemic rats. AG extract decreased the increase of BBB permeability, and neuronal death and inhibited the activation of astrocytes and microglia in ischemic brains. AG extract also significantly increased the expression of Ang-1, Tie-2, VEGF, ZO-1 and Occludin through activation of the PI3K/Akt pathway. AG extract significantly increased the expression of ICAM-1 in ischemic brains.
Our results indicate that the hairy root of AG has a neuroprotective effect in ischemic stroke.
在本研究中，我们通过调节血管生成分子，研究了当归毛状根提取物对大鼠短暂性局灶性脑缺血的神经保护作用。
雄性Sprague-Dawley大鼠通过短暂大脑中动脉闭塞（tMCAO）诱导局灶性脑缺血90分钟，然后口服当归毛状根水提取物（AG）。再灌注24小时后，通过TTC和伊文思蓝（EB）染色测量梗死体积和血脑屏障通透性的变化。通过免疫组织化学评估缺血脑中神经元细胞损伤和胶质细胞的激活。分别通过蛋白质印迹法测定缺血脑中血管生成诱导蛋白如血管生成素-1（Ang-1）和血管内皮生长因子（VEGF）、炎症蛋白如细胞间粘附分子-1（CAM-1）、紧密连接蛋白如ZO-1和闭合蛋白以及磷脂酰肌醇3激酶（PI3K）/AKT的磷酸化水平。
AG提取物治疗显著降低了MACO诱导的缺血大鼠的脑梗死体积和水肿。AG提取物降低了血脑屏障通透性的增加和神经元死亡，并抑制了缺血脑中星形胶质细胞和小胶质细胞的激活。AG提取物还通过激活PI3K/Akt途径显著增加了Ang-1、Tie-2、VEGF、ZO-1和闭合蛋白的表达。AG提取物显著增加了缺血脑中ICAM-1的表达。
我们的结果表明，当归毛状根对缺血性中风具有神经保护作用。
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