National Institute for Health Research, Southampton Biomedical Research Centre, University of Southampton and University Hospital Southampton NHS Foundation Trust, Southampton, United Kingdom. Human Development and Health Academic Unit, Faculty of Medicine, University of Southampton, Southampton, United Kingdom.
J Breath Res. 2018 Jul 19;12(4):046002. doi: 10.1088/1752-7163/aacf12.
Hepatic mitochondrial function (HMF) assessed by the C-ketoisocaproate breath test (C-KICA BT) has been previously shown to be significantly associated with the severity of biopsy proven non-alcoholic fatty liver disease (NAFLD). However, it is uncertain whether any perturbation in HMF relates specifically to severity of liver disease or factors associated with metabolic syndrome within (NAFLD). Our aim was to investigate whether there was any change in HMF assessed by C-KICA BT in patients with NAFLD compared to control subjects, and to assess the factors that are independently associated with HMF.
77 patients with NAFLD and 11 healthy control subjects were studied. HMF was assessed using C-KICA BT and expressed as cumulative % C-dose recovered on breath over 1 h (cPDR over 1 h). Liver fat and fibrosis was assessed by transient elastography. Multivariable linear regression modelling was undertaken to test the independence of associations with HMF.
HMF (cPDR over 1 h) was lower in NAFLD compared to controls [13.4% (4.8) v. 21.0% (6.3); p < 0.0001)]. In NAFLD, HMF was lower in patients with diabetes versus no diabetes [12.7% (3.4) v. 14.3% (6.1); p = 0.003)]. Regression modelling showed age (β = -0.08; p = 0.01), waist circumference (β = -0.08; p = 0.01), hip circumference (β = -0.04; p = 0.01), aspartate aminotransferase (AST) (β = -0.05; p = 0.01) and diabetes status (β = -1.81; p = 0.01) were independently associated with HMF (R = 41.5%; p < 0.0001).
In patients with NAFLD (compared to healthy subjects), there was a reduction in HMF assessed by the C-KICA BT. Furthermore, in patients with NAFLD, HMF is independent and inversely associated with age, waist and hip circumference, AST and diabetes status.
研究非酒精性脂肪性肝病（NAFLD）患者与健康对照者相比，通过 C-酮异己酸呼吸试验（C-KICA BT）评估的肝线粒体功能（HMF）是否发生变化，并评估与 HMF 独立相关的因素。
研究了 77 例 NAFLD 患者和 11 例健康对照者。采用 C-KICA BT 评估 HMF，以 1 小时内呼气中累积 C-剂量恢复率（cPDR 超过 1 小时）表示。通过瞬态弹性成像评估肝脂肪和纤维化。采用多元线性回归模型检验与 HMF 相关的独立性。
与对照组相比，NAFLD 患者的 HMF（cPDR 超过 1 小时）较低[13.4%（4.8）比 21.0%（6.3）；p<0.0001)]。在 NAFLD 中，与无糖尿病相比，糖尿病患者的 HMF 较低[12.7%（3.4）比 14.3%（6.1）；p=0.003)]。回归模型显示年龄（β=-0.08；p=0.01）、腰围（β=-0.08；p=0.01）、臀围（β=-0.04；p=0.01）、天门冬氨酸氨基转移酶（AST）（β=-0.05；p=0.01）和糖尿病状态（β=-1.81；p=0.01）与 HMF 独立相关（R=41.5%；p<0.0001）。
与健康受试者相比，NAFLD 患者的 C-KICA BT 评估的 HMF 降低。此外，在 NAFLD 患者中，HMF 与年龄、腰围和臀围、AST 和糖尿病状态独立且呈负相关。
