Skog S, Tribukait B, Sundius G
Acta Radiol Oncol. 1985 Jul-Aug;24(4):375-82. doi: 10.3109/02841868509136068.
The effects of roentgen irradiation on the incorporation of 3H-uridine and 14C-leucine into RNA and protein and the RNA and protein contents of in vivo growing Ehrlich ascites tumour cells were studied. The results were related to changes in the composition of cells in cell cycle and compared with the synthesis of RNA and protein in cell material from various parts of the cell cycle obtained by means of elutriator centrifuging. The incorporation expressed by the ratio between acid insoluble/acid soluble activity was unchanged for RNA during the observation period up to 24 hours after a dose of 5.0 Gy. The ratio for protein was markedly decreased between 4 and 24 hours. This decrease was partly due to a decrease of the pool size of leucine as studied by changing the amounts of 14C leucine used. From these studies, the existence of at least two pools, an expandable and a non-expandable fixed pool can be concluded. There were no differences in the decrease of protein-synthesis between cells from the various parts of the cell cycle. The RNA and protein contents of the irradiated cells from various parts of the cell cycle corresponded to those of non-irradiated cells except for G1/early S-phase cells at 15 and 24 hours after irradiation. Possible reasons for this discrepancy are discussed.
研究了X射线照射对体内生长的艾氏腹水瘤细胞中3H-尿苷和14C-亮氨酸掺入RNA和蛋白质以及RNA和蛋白质含量的影响。将结果与细胞周期中细胞组成的变化相关联，并与通过淘洗离心从细胞周期各个部分获得的细胞物质中RNA和蛋白质的合成进行比较。在5.0 Gy剂量后长达24小时的观察期内，以酸不溶性/酸溶性活性之比表示的RNA掺入量没有变化。蛋白质的该比例在4至24小时之间明显降低。通过改变所使用的14C亮氨酸的量进行研究，这种降低部分是由于亮氨酸库大小的减小。从这些研究中，可以得出结论，至少存在两个库，一个可扩展的和一个不可扩展的固定库。细胞周期各个部分的细胞之间蛋白质合成的降低没有差异。除了照射后15和24小时的G1/早期S期细胞外，来自细胞周期各个部分的受照射细胞的RNA和蛋白质含量与未受照射细胞的含量相对应。讨论了这种差异的可能原因。
