Skog S, Tribukait B, Sundius G
Acta Radiol Oncol. 1983;22(5):369-79. doi: 10.3109/02841868309134055.
The cell-cycle related energy metabolism after roentgen irradiation of in vivo growing Ehrlich ascites tumour cells was investigated in cell fractions obtained by elutriator centrifuging. The oxygen consumption and the lactate and pyruvate production, measured in vitro after 4.5, 5 and 9 Gy up to 24 h, were undisturbed, while the decrease in the in vivo ATP content was dose-independent in all parts of the cell cycle. On the basis of these data the ATP turnover time was found to be decreased. The decrease in the ATP content is considered to be less likely to be due to membrane leakage or increased ATP consumption than to reduced ATP production. Since in vitro incubation normalizes the ATP content, it is suggested that environmental factors in the ascites liquid after irradiation cause a decrease in the ATP production. Low ATP contents of the cells do not appear to influence the irradiation-induced changes in the cell flow through the cell cycle.
通过淘洗离心获得细胞组分，研究体内生长的艾氏腹水瘤细胞经X射线照射后与细胞周期相关的能量代谢。在4.5、5和9戈瑞照射后长达24小时进行体外测量，发现氧气消耗以及乳酸和丙酮酸生成均未受干扰，而细胞周期各阶段体内ATP含量的降低与剂量无关。基于这些数据，发现ATP周转时间缩短。ATP含量的降低被认为不太可能是由于膜渗漏或ATP消耗增加，而更可能是由于ATP生成减少。由于体外孵育可使ATP含量恢复正常，因此提示照射后腹水中的环境因素导致ATP生成减少。细胞的低ATP含量似乎不影响照射诱导的细胞周期中细胞流动的变化。
