Sanchis-Perucho P, Duran F, Barat R, Pachés M, Aguado D
CALAGUA - Unidad Mixta UV-UPV, Institut Universitari d'Investigació d'Enginyeria de l'Aigua i Medi Ambient - IIAMA, Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain E-mail:
Water Sci Technol. 2018 Jun;77(11-12):2566-2577. doi: 10.2166/wst.2018.207.
The aim of this study was to evaluate the effect of light intensity and phosphorus concentration on biomass growth and nutrient removal in a microalgae culture and their effect on their competition. The photobioreactor was continuously fed with the effluent from an anaerobic membrane bioreactor pilot plant treating real wastewater. Four experimental periods were carried out at different light intensities (36 and 52 μmol s m) and phosphorus concentrations (around 6 and 15 mgP L). Four green algae - Scenedesmus, Chlorella, Monoraphidium and Chlamydomonas- and cyanobacterium were detected and quantified along whole experimental period. Chlorella was the dominant species when light intensity was at the lower level tested, and was competitively displaced by a mixed culture of Scenedesmus and Monoraphidium when light was increased. When phosphorus concentration in the photobioreactor was raised up to 15 mgP L, a growth of cyanobacterium became the dominant species in the culture. The highest nutrient removal efficiency (around 58.4 ± 15.8% and 96.1 ± 16.5% of nitrogen and phosphorus, respectively) was achieved at 52 μmol s m of light intensity and 6.02 mgP L of phosphorus concentration, reaching about 674 ± 86 mg L of volatile suspended solids. The results obtained reveal how the light intensity supplied and the phosphorus concentration available are relevant operational factors that determine the microalgae species that is able to predominate in a culture. Moreover, changes in microalgae predominance can be induced by changes in the growth medium produced by the own predominant species.
本研究的目的是评估光强和磷浓度对微藻培养中生物量增长和养分去除的影响及其对竞争的影响。光生物反应器连续进料来自处理实际废水的厌氧膜生物反应器中试装置的出水。在不同光强（36和52 μmol s⁻¹ m⁻²）和磷浓度（约6和15 mgP L⁻¹）下进行了四个实验阶段。在整个实验期间对四种绿藻——栅藻、小球藻、单针藻和衣藻——以及蓝细菌进行了检测和定量。当光强处于测试的较低水平时，小球藻是优势种，而当光强增加时，它被栅藻和单针藻的混合培养物竞争性取代。当光生物反应器中的磷浓度提高到15 mgP L⁻¹时，蓝细菌的生长成为培养物中的优势种。在光强为52 μmol s⁻¹ m⁻²和磷浓度为6.02 mgP L⁻¹时实现了最高的养分去除效率（分别约为氮和磷的58.4 ± 15.8%和96.1 ± 16.5%），挥发性悬浮固体达到约674 ± 86 mg L⁻¹。获得的结果揭示了所提供的光强和可用的磷浓度是决定能够在培养物中占主导地位的微藻种类的相关操作因素。此外，微藻优势的变化可以由自身优势种产生的生长培养基变化诱导。
