Eom H, Lee I
a School of Biological Sciences , Seoul National University , Seoul Korea.
b Center for RNA Research , Institute for Basic Science , Seoul , Korea.
Plant Signal Behav. 2018;13(7):e1471300. doi: 10.1080/15592324.2018.1471300. Epub 2018 Jun 26.
The autonomous pathway promotes flowering by repressing a major flowering repressor, FLOWERING LOCUS C (FLC). Approximately 30 genes are involved in this pathway, and several of them are related to RNA processing; however, the molecular basis of the transcriptional regulation of FLC is yet to be understood. Recently, we discovered a new autonomous pathway gene, TATA-binding protein-associated factor 15b (TAF15b), which has a RNA recognition motif (RRM) and represses the level of FLC transcripts. TAF15b regulates the expression of FLC by directly interacting with RNA polymerase II (Pol II) at the transcription start sites on both the sense and antisense strands of the FLC locus. In addition to the transcriptional regulation in the nucleus, TAF15b accumulates in processing bodies (p-bodies), which are cytoplasmic RNA granules involved in translational repression, during heat stress. Here we discuss the implications of our findings and suggest a dual role of TAF15b in both transcriptional and translational regulation.
自主途径通过抑制主要的开花抑制因子开花位点C（FLC）来促进开花。大约30个基因参与该途径，其中一些与RNA加工有关；然而，FLC转录调控的分子基础尚不清楚。最近，我们发现了一个新的自主途径基因，即TATA结合蛋白相关因子15b（TAF15b），它具有一个RNA识别基序（RRM）并抑制FLC转录本的水平。TAF15b通过在FLC基因座的正义链和反义链的转录起始位点与RNA聚合酶II（Pol II）直接相互作用来调节FLC的表达。除了在细胞核中的转录调控外，在热胁迫期间，TAF15b还会在加工小体（p小体）中积累，加工小体是参与翻译抑制的细胞质RNA颗粒。在此，我们讨论了我们研究结果的意义，并提出了TAF15b在转录和翻译调控中的双重作用。
