Department of Virology, National AIDS Research Institute, Pune, India.
National Institute of Virology, Pune, India.
Microb Pathog. 2018 Oct;123:82-88. doi: 10.1016/j.micpath.2018.06.035. Epub 2018 Jun 23.
Herpes simplex virus type-2 (HSV-2) is an important sexually transmitted pathogen that infects the genital mucosal epithelial cells causing ulcerative lesions at the site of entry, facilitating HIV infection. The infection of epithelial cells and skin resident dendritic cells with HSV-2 causes a release of chemokine and retinoic acid which attracts CD4 T-cells to the genital mucosa. In this study, we investigated whether HSV-2 (ATCC VR734) could infect and replicate in two T-cell lines (CEM CCR5+ and MOLT4 CCR5+). The growth of HSV-2 was assessed by plaque assay while the intracellular HSV-2 was identified using infectious center and indirect immunofluorescence assays. The replication of HSV-2 in T-cell lines was compared to a cell line (Vero) which is routinely used for growing HSV-2. Analysis indicated that a low level of infection was detected in the two T-cells lines and was dependent on the infectious dose as well as the time of adsorption. Indirect immunofluorescence showed presence of HSV-2 antigens in the CEM CCR5+ and Vero cell lines but not in MOLT4 CCR5+. The data suggests that T-cells can support growth of HSV-2 which might contribute to changes in gene expression of T-cells. This is an important aspect that needs to be further investigated in relation of HIV-1/HSV-2 viral synergy.
单纯疱疹病毒 2 型（HSV-2）是一种重要的性传播病原体，感染生殖器黏膜上皮细胞，在感染部位引起溃疡性病变，促进 HIV 感染。HSV-2 感染上皮细胞和皮肤常驻树突状细胞会释放趋化因子和视黄酸，吸引 CD4 T 细胞到生殖器黏膜。在这项研究中，我们研究了 HSV-2（ATCC VR734）是否可以感染和复制两种 T 细胞系（CEM CCR5+和 MOLT4 CCR5+）。通过噬斑测定评估 HSV-2 的生长，通过感染中心和间接免疫荧光测定鉴定细胞内 HSV-2。将 HSV-2 在 T 细胞系中的复制与常规用于生长 HSV-2 的细胞系（Vero）进行比较。分析表明，在两种 T 细胞系中检测到低水平的感染，这取决于感染剂量和吸附时间。间接免疫荧光显示 CEM CCR5+和 Vero 细胞系中存在 HSV-2 抗原，但 MOLT4 CCR5+中不存在。数据表明，T 细胞可以支持 HSV-2 的生长，这可能导致 T 细胞基因表达的变化。这是与 HIV-1/HSV-2 病毒协同作用相关的一个需要进一步研究的重要方面。
