Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
Institute of Plant Quarantine, Ministry of Agriculture, Sichuan Province, 610041, China.
Int J Biol Macromol. 2018 Oct 15;118(Pt A):1229-1237. doi: 10.1016/j.ijbiomac.2018.06.099. Epub 2018 Jun 23.
Bactrocera tsuneonis (Miyake), generally known as the Japanese orange fly, is considered to be a major pest of commercial citrus crops. It has a limited distribution in China, Japan and Vietnam, but it has the potential to invade areas outside of Asia. More genetic information of B. tsuneonis should be obtained in order to develop effective methodologies for rapid and accurate molecular identification due to the difficulty of distinguishing it from Bactrocera minax based on morphological features. We report here the whole mitochondrial genome of B. tsuneonis sequenced by next-generation sequencing. This mitogenome sequence had a total length of 15,865 bp, a typical circular molecule comprising 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a non-coding region (A + T-rich control region). The structure and organization of the molecule were typical and similar compared with the published homologous sequences of other fruit flies in Tephritidae. The phylogenetic analyses based on the mitochondrial genome data presented a close genetic relationship between B. tsuneonis and B. minax. This is the first report of the complete mitochondrial genome of B. tsuneonis, and it can be used in further studies of species diagnosis, evolutionary biology, prevention and control.
黄斑长翅卷叶蛾（Miyake），通常被称为日本橘实蝇，被认为是商业柑橘作物的主要害虫。它在中国、日本和越南的分布有限，但有可能入侵亚洲以外的地区。由于基于形态特征难以将其与小眼实蝇区分开来，因此需要获得更多的黄斑长翅卷叶蛾遗传信息，以便开发快速准确的分子鉴定方法。我们在此报告通过下一代测序获得的黄斑长翅卷叶蛾的整个线粒体基因组序列。该线粒体基因组全长 15865bp，是一个典型的圆形分子，包含 13 个蛋白质编码基因、2 个 rRNA 基因、22 个 tRNA 基因和一个非编码区（富含 A+T 的调控区）。与其他双翅目实蝇科已发表的同源序列相比，该分子的结构和组织具有典型性和相似性。基于线粒体基因组数据的系统发育分析表明，黄斑长翅卷叶蛾与小眼实蝇之间存在密切的遗传关系。这是黄斑长翅卷叶蛾完整线粒体基因组的首次报道，可用于物种诊断、进化生物学、预防和控制的进一步研究。
