Liang Tingting, Han Zhifeng, Zhao Huan, Zhang Xuan, Wang Yao
Clin Lab. 2018 Jun 1;64(6):895-900. doi: 10.7754/Clin.Lab.2018.171007.
Our previous studies revealed that concentrations of circulating antibodies to annexin A1 (ANXA1) were increased in non-small lung cancer (NSCLC). This study was thus designed to replicate this initial finding with an independent sample set.
An enzyme-linked immunosorbent assay (ELISA) was developed in-house to examine plasma antiANXA1 IgG levels in 220 patients with NSCLC and 200 control subjects.
Mann-Whitney U test showed that patients with NSCLC had significantly higher anti-ANXA1 IgG levels than control subjects (Z = -4.02, p < 0.001); male patients appeared to mainly contribute to the increased antibody level (Z = -3.09, p = 0.002). Receiver operating characteristic (ROC) curve analysis showed an overall area under the ROC curve (AUC) of 0.61 (95% CI: 0.56 - 0.67), with sensitivity of 8% against a specificity of 95.0%. Spearman's correlation analysis failed to show a significant correlation between the anti-ANXA1 IgG levels and the expression of three tumor-associated antigens including p53 (r = 0.156, p = 0.027), Ki67 (r = -0.048, p = 0.489), and EGFR (r = 0.02, p = 0.782).
Increased levels of circulating anti-ANXA1 IgG antibody may have a prognostic value for NSCLC.
我们之前的研究表明，非小细胞肺癌（NSCLC）患者循环中膜联蛋白A1（ANXA1）抗体浓度升高。因此，本研究旨在用独立样本集重复这一初步发现。
自行开发酶联免疫吸附测定（ELISA）法，检测220例NSCLC患者和200例对照者血浆中抗ANXA1 IgG水平。
曼-惠特尼U检验显示，NSCLC患者的抗ANXA1 IgG水平显著高于对照者（Z = -4.02，p < 0.001）；男性患者似乎是抗体水平升高的主要原因（Z = -3.09，p = 0.002）。受试者工作特征（ROC）曲线分析显示，ROC曲线下总面积（AUC）为0.61（95%CI：0.56 - 0.67），敏感性为8%，特异性为95.0%。Spearman相关性分析未显示抗ANXA1 IgG水平与包括p53（r = 0.156，p = 0.027）、Ki67（r = -0.048，p = 0.489）和EGFR（r = 0.02，p = 0.782）在内的三种肿瘤相关抗原的表达之间存在显著相关性。
循环抗ANXA1 IgG抗体水平升高可能对NSCLC具有预后价值。
