Huang Yile, Zhang Cong, Chen Cairen, Sun Shilong, Zheng He, Wan Shuli, Meng Qingyong, Chen Yubin, Wei Jun
School of Clinical Laboratory Science, Guangdong Medical College, Dongguan, 523808, Guangdong, China.
Tumour Biol. 2015 Feb;36(2):1233-6. doi: 10.1007/s13277-014-2751-x. Epub 2014 Oct 25.
Our recent work demonstrated that circulating levels of IgG antibody to linear peptide antigens derived from annexin A1 (ANXA1) were significantly increased in lung cancer. The present study was then undertaken to test whether circulating anti-ANXA1 antibodies were also altered in breast cancer. An enzyme-linked immunosorbent assay was developed in-house to determine circulating IgG against ANXA1-derived peptide antigens in 152 female patients with breast cancer and 160 female control subjects. Student's t test revealed that patients with breast cancer had significantly higher levels of anti-ANXA1 IgG than control subjects (t = 4.75, P < 0.0001). Receiver operating characteristic (ROC) analysis showed that the area under the ROC curve was 0.73 with 95% confidence interval (CI) 0.67-0.78, and the sensitivity of anti-ANXA1 IgG assay was 23.2% against the specificity of 90%. The levels of anti-ANXA1 IgG did not appear to be stage-dependent, and Pearson correlation analysis showed no correlation between the anti-ANXA1 IgG levels and the stages of breast cancer (r = -0.02, df = 149, P = 0.796). This work suggests that circulating IgG for ANXA1-derived peptide antigens may have both diagnostic and prognostic values for breast cancer although further screening is needed to identify more such peptide antigens derived from tumor-associated antigens.
我们最近的研究表明，肺癌患者中抗膜联蛋白A1（ANXA1）线性肽抗原的循环IgG抗体水平显著升高。本研究旨在检测乳腺癌患者的循环抗ANXA1抗体是否也发生改变。我们自行开发了一种酶联免疫吸附测定法，以测定152例女性乳腺癌患者和160例女性对照受试者血清中抗ANXA1衍生肽抗原的IgG水平。学生t检验显示，乳腺癌患者的抗ANXA1 IgG水平显著高于对照组（t = 4.75，P < 0.0001）。受试者工作特征（ROC）分析表明，ROC曲线下面积为0.73，95%置信区间（CI）为0.67 - 0.78，抗ANXA1 IgG检测的敏感性为23.2%，特异性为90%。抗ANXA1 IgG水平似乎与分期无关，Pearson相关分析显示抗ANXA1 IgG水平与乳腺癌分期之间无相关性（r = -0.02，自由度 = 149，P = 0.796）。这项研究表明，尽管需要进一步筛选以鉴定更多来源于肿瘤相关抗原的此类肽抗原，但抗ANXA1衍生肽抗原的循环IgG对乳腺癌可能具有诊断和预后价值。
