Deng Jiu-Ling, Chen Liang, Shou Qi-Yang, Luo Qi-Han, Wang Hui-Ming, Wang Hui
Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China.
Zhongguo Zhong Yao Za Zhi. 2018 Jun;43(11):2372-2377. doi: 10.19540/j.cnki.cjcmm.20180327.004.
To investigate the protective effect of Yiqi Huoxue decoction on the kidney in diabetic goto-Kaizaki (GK) rats. Eight Wistar rats were used as normal control group. According to body weight and blood glucose, 16 GK rats were randomly divided into model control group and Yiqi Huoxue group, with 8 rats in each group. Drugs were administrated for 6 weeks. The rats were weighed and fasting blood glucose (FBG) were measured weekly; the glycosylated hemoglobin (HbALc) and 24 h metabolic rate were measured at the end of third and sixth weeks, at the same time, urine was collected to measure urinary glucose (U-GLU), urinary creatinine (U-CREA) and total proteinuria (U-TP) by biochemical method and calculate 24 h protein excretion rate (UAER). At the end of treatment, rats were sacrificed in anesthesia, and creatinin (CREA), uric acid (UA) and cholesterol (CHOL) were detected by biochemical method and glutathione peroxidase (GSH-Px) was detected by manual method with the blood drawn from heart. Both kidneys were taken and weighed to calculate the renal hypertrophy index. The kidneys were placed into neutral formalin and observed under light microscope for pathological change after Periodic Acid-Schiff (PAS) staining. The results showed that as compared with the normal control group, FBG, HbALc, 24 h Metabolic rate, U-GLU, UAER, serum CREA, UA and CHOL levels, as well as renal hypertrophy index were significantly increased (<0.05) in the model group, while the body weight growth rate, serum GSH-Px level were significantly reduced(<0.05); in addition, renal glomerular injury and structural changes were observed in the model group. As compared with the model group, serum GSH-Px level was significantly increased (<0.05) in the Yiqi Huoxue group, While FBG, HbALc, 24 h Metabolic rate, U-GLU, UAER, serum CREA, UA and CHOL levels, as well as renal hypertrophy index were significantly reduced (<0.05); meanwhile, renal glomerular injury and structural changes were improved in the Yiqi Huoxue group. The results showed that Yiqi Huoxue decoction can significantly increase serum GSH-Px level, reduce FBG, HbALc, 24 h metabolic rate, U-GLU, UAER, serum CREA, UA and CHOL levels, as well as renal hypertrophy index, and improve renal pathology in diabetic GK rats.
探讨益气活血汤对糖尿病Goto-Kaizaki（GK）大鼠肾脏的保护作用。选取8只Wistar大鼠作为正常对照组。将16只GK大鼠根据体重和血糖随机分为模型对照组和益气活血组，每组8只。给药6周。每周称大鼠体重并测量空腹血糖（FBG）；在第3周和第6周结束时测量糖化血红蛋白（HbALc）和24小时代谢率，同时收集尿液，采用生化方法测定尿葡萄糖（U-GLU）、尿肌酐（U-CREA）和总蛋白尿（U-TP），并计算24小时尿蛋白排泄率（UAER）。治疗结束时，将大鼠麻醉处死，采用生化方法检测肌酐（CREA）、尿酸（UA）和胆固醇（CHOL），并采用手工方法从心脏采血检测谷胱甘肽过氧化物酶（GSH-Px）。取出双侧肾脏称重，计算肾脏肥大指数。将肾脏置于中性福尔马林中，经高碘酸-希夫（PAS）染色后在光学显微镜下观察病理变化。结果显示，与正常对照组相比，模型组的FBG、HbALc、24小时代谢率、U-GLU、UAER、血清CREA、UA和CHOL水平以及肾脏肥大指数均显著升高（<0.05），而体重增长率、血清GSH-Px水平显著降低（<0.05）；此外，模型组观察到肾小球损伤和结构改变。与模型组相比，益气活血组血清GSH-Px水平显著升高（<0.05），而FBG、HbALc、24小时代谢率、U-GLU、UAER、血清CREA、UA和CHOL水平以及肾脏肥大指数均显著降低（<0.05）；同时，益气活血组肾小球损伤和结构改变得到改善。结果表明，益气活血汤可显著提高糖尿病GK大鼠血清GSH-Px水平，降低FBG、HbALc、24小时代谢率、U-GLU、UAER、血清CREA、UA和CHOL水平以及肾脏肥大指数，并改善肾脏病理。
