Li Ying, Wang Lei-Sha, Liu Jian-Xun, Song Wen-Ting, Xu Li, Li Hong-Hai, Li Jun-Mei, Yang Bin, Dong Xiao-Xia, Wang Guang-Rui, Yao Ming-Jiang, Lin Cheng-Ren
Beijing University of Chinese Medicine, Beijing 100029, China.
Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing Key Laboratory of Pharmacology of Chinese Materia Medica, Beijing 100091, China.
Zhongguo Zhong Yao Za Zhi. 2018 Feb;43(4):786-793. doi: 10.19540/j.cnki.cjcmm.20171027.023.
This study aimed to observe the general state and changes in pathophysiological indexes of multiple cerebral infarction rat model with Qi-deficienty and Blood-stasis syndrome. Rats were randomly divided into 4 groups(with 30 in each group): the normal group, the sham group, the model group and the Yiqi Huoxue recipe group. Rats in the model group and Yiqi Huoxue group were provided with interruptable sleep deprivation for 7 days before the multiple cerebral infarction operation, and followed by another 4 weeks of sleep deprivation; rats in the Yiqi Huoxue group were intragastrically administrated with drug at a dose of 26 g·kg⁻¹, once a day for 4 weeks. The general state was observed, and the pathophysiological indexes were measured at 48 h, 2 weeks and 4 weeks after administration. The results showed that rats in the normal group and the sham group represented a good general state and behaviors, with a normal morphological structure of brain tissues; rats in the model group featured yellow fur, depression, accidie, loose stools and movement disorder, with obvious brain histomorphological damage, which became aggravated with the increase of modeling time; rats in the Yiqi Huoxue group showed release in the general state and above indexes. Compared with the sham group at three time points, rats in the model group showed decrease in body weight, exhaustive swimming time and RGB value of tongue surface image, and increase in whole blood viscosity of the shear rate under 5, 60 and 150 S⁻¹, reduction in cerebral cortex Na⁺-K⁺-ATPase, Ca²⁺-ATPase activity and contents of 5-HT, rise in TXB2 levels and decline in 6-keto-PGF1a in serum(<0.05, <0.01). Compared with the model group, rats in the Yiqi Huoxue group showed alleviations in the above indexes at 2 w and 4 w(<0.05, <0.01). The results showed that the characterization and pathophysiological indexes in the multiple cerebral infarction rat model with Qi-deficiency and blood-stasis syndrome were deteriorated; Yiqi Huoxue recipe could significantly alliviate the abnormal conditions, which suggested of the model was stable and reliable and the pathophysiologic evolutionary mechanism might be related to energy metabolism dysfunction, vasoactive substance abnormality and changes in neurotransmitters.
本研究旨在观察气虚血瘀证多发性脑梗死大鼠模型的一般状态及病理生理指标变化。将大鼠随机分为4组（每组30只）：正常组、假手术组、模型组和益气活血方组。模型组和益气活血组大鼠在多发性脑梗死手术前进行7天的间断性睡眠剥夺，随后再进行4周的睡眠剥夺；益气活血组大鼠按26 g·kg⁻¹剂量灌胃给药，每日1次，共4周。观察一般状态，并在给药后48小时、2周和4周测量病理生理指标。结果显示，正常组和假手术组大鼠一般状态及行为良好，脑组织形态结构正常；模型组大鼠毛发发黄、精神抑郁、倦怠、便溏、运动障碍，脑组 织形态学损伤明显，且随造模时间延长而加重；益气活血组大鼠上述一般状态及指标有所缓解。与假手术组在三个时间点比较，模型组大鼠体重、力竭游泳时间、舌面图像RGB值降低，5、60和150 S⁻¹切变率下全血黏度升高，大脑皮质Na⁺-K⁺-ATP酶、Ca²⁺-ATP酶活性及5-HT含量降低，血清TXB2水平升高、6-keto-PGF1a降低（<0.05，<0.01）。与模型组比较，益气活血组大鼠在2周和4周时上述指标有所改善（<0.05，<0.01）。结果表明，气虚血瘀证多发性脑梗死大鼠模型的表征及病理生理指标恶化；益气活血方可明显改善异常状况，提示该模型稳定可靠，其病理生理演变机制可能与能量代谢障碍、血管活性物质异常及神经递质变化有关。
