Wang Cong, Li Yongwen, Li Ying, Gong Hao, Zhang Hongbing, Yuan Yin, Li Weiting, Liu Hongyu, Chen Jun
Department of Lung Cancer Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China.
Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China.
Zhongguo Fei Ai Za Zhi. 2018 Jun 20;21(6):445-450. doi: 10.3779/j.issn.1009-3419.2018.06.04.
LC-3 and P62, two of important autophagy-related proteins, were reported highly expressed in many kinds of human malignancies and associated with outcomes of the patients. The purpose of this study was to investigate the expression status of LC-3 and P62 in non-small cell lung cancer patients and define the clinical-pathologic features.
66 cases of non-small cell lung cancer patients were employed. The expression of LC-3 and P62 were detected by immunohistochemistry.
LC-3 was positive stained in 27 out of 66 cases (40.9%) and P62 was positive stained in 43 out of 66 cases (65.2%). LC-3 positive staining was more frequently in squamous cell carcinoma patients (P<0.05); while P62 positive staining was more frequently in late-stage adenocarcinoma patients with metastasis (P<0.05). There was a significant negative correlation between LC-3 and P62 expressions in non-small cell lung cancer tissues (rs=-0.065, P<0.001). Kaplan-Meier analysis showed that patients with positive LC-3 expression had favorable clinical outcomes compared with the patients with negative LC-3 expression (P<0.05).
LC-3 and P62 showed abnormal expression in non-small cell lung cancer tissues, suggesting that autophagy is involved in the occurrence and development of NSCLC.
LC-3和P62是两种重要的自噬相关蛋白，据报道在多种人类恶性肿瘤中高表达，并与患者的预后相关。本研究旨在调查非小细胞肺癌患者中LC-3和P62的表达情况，并明确其临床病理特征。
纳入66例非小细胞肺癌患者。采用免疫组织化学法检测LC-3和P62的表达。
66例患者中，27例（40.9%）LC-3呈阳性染色，43例（65.2%）P62呈阳性染色。LC-3阳性染色在鳞状细胞癌患者中更为常见（P<0.05）；而P62阳性染色在有转移的晚期腺癌患者中更为常见（P<0.05）。在非小细胞肺癌组织中，LC-3和P62的表达呈显著负相关（rs=-0.065，P<0.001）。Kaplan-Meier分析显示，与LC-3表达阴性的患者相比，LC-3表达阳性的患者临床预后较好（P<0.05）。
LC-3和P62在非小细胞肺癌组织中表达异常，提示自噬参与了非小细胞肺癌的发生和发展。
